制何首乌

中文文本参考：《中国药典（2020年版）》
English text reference: Chinese Pharmacopoeia (2020 Edition)

概述 Overview

本品为何首乌的炮制加工品。
Prepared Fleeceflower Root is the processed product of the root of Polygonum multiflorum Thunb. (Fam. Polygonaceae).

炮制 Processing

取何首乌片或块，照炖法（通则0213）用黑豆汁拌匀，置非铁质的适宜容器内，炖至汁液吸尽；或照蒸法（通则 0213），清蒸或用黑豆汁拌匀后蒸，蒸至内外均呈棕褐色，或晒至半干，切片，干燥。
Take the slices or blocks of Prepared Fleeceflower Root, mix them with black bean juice according to the stewing method <0213>, place them in a suitable non-ferrous container, stew until the juice is absorbed; or steam them according to the steaming method <0213>, steam them after mixing with black bean juice, steam until both the inside and outside are brown, or dry them until semi-dry, slice and dry.

每100kg何首乌片（块），用黑豆10kg。
For every 100kg of Prepared Fleeceflower Root slices (blocks), use 10kg of black beans.

黑豆汁制法 Black bean juice method

取黑豆10kg，加水适量，煮约4小时，熬汁约15kg，豆渣再加水煮约3小时，熬汁约10kg，合并得黑豆汁约25kg。
Method for making black bean juice: Take 10kg of black beans, add an appropriate amount of water, boil for about 4 hours, simmer to obtain about 15kg of juice, boil the bean dregs with water for about 3 hours, simmer to obtain about 10kg of juice, combine to obtain about 25kg of black bean juice.

性状 Description

本品呈不规则皱缩状的块片，厚约1cm。表面黑褐色或棕褐色，凹凸不平。质坚硬，断面角质样，棕褐色或黑色。气微，味微甘而苦涩。
Prepared Fleeceflower Root is irregularly wrinkled and shriveled, about 1cm thick. The surface is blackish-brown or brownish-brown, uneven. The texture is hard, the section is horny, brownish-brown or black. It has a slight odor, and a slightly sweet and bitter taste.

鉴别 Identification

照何首乌项下的〔鉴别〕（2）项试验，显相同的结果。
Perform the test under the [Identification] (2) item of Prepared Fleeceflower Root, and obtain the same results.

检查 Examination

水分 Water

不得过12.0%（通则0832第二法）。
Not more than 12.0 per cent <0832,method 2>.

总灰分 Total ash

不得过9.0%（通则2302）。
Not more than 9.0 per cent <2302>.

浸出物 Extractives

照醇溶性浸出物测定法（通则2201）项下的热浸法测定，用乙醇作溶剂，不得少于5.0%。
Carry out the method for determination of ethanol-soluble Extractives <2201,the hot maceration method>, using ethanol as the solvent,not less than 5.0 per cent.

含量测定 Content determination

二苯乙烯苷 Emodin-8-O-glucoside

避光操作。
Handle in the dark.

取本品粉末（过四号筛）约0.2g，精密称定，照何首乌药材〔含量测定〕项下的方法测定。
Take about 0.2g of the powder (passed through a No. 4 sieve) of this material, accurately weigh, and determine according to the method under "Content determination" of Polygonum multiflorum.

本品按干燥品计算，含2，3，5，4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷（C20H22O9）不得少于0. 70%。
Calculated on the dried basis, the content of emodin-8-O-glucoside (C20H22O9) shall not be less than 0.70%.

游离蒽醌 Free anthraquinones

照高效液相色谱法（通则0512）测定。
Determine by high-performance liquid chromatography <0512>.

色谱条件与系统适用性试验 Chromatographic conditions and system suitability tests

以十八烷基硅烷键合硅胶为填充剂；以甲醇-0.1%磷酸溶液（80： 20）为流动相；检测波长为254nm。理论板数按大黄素峰计算应不低于3000。
The chromatographic conditions and system suitability test: using octadecylsilane-bonded silica gel as the filler; using methanol-0.1% phosphoric acid solution (80:20) as the mobile phase; the detection wavelength is 254nm. The theoretical plate number calculated based on the peak of emodin should not be less than 3000.

对照品溶液的制备 Preparation of the reference solution

取大黄素对照品、大黄素甲醚对照品适量，精密称定，加甲醇分别制成每1ml含大黄素80μg、大黄素甲醚40μg的溶液，即得。
Preparation of reference solution: Take an appropriate amount of emodin reference substance and emodin methyl ether reference substance, accurately weigh, and add methanol to prepare a solution containing 80μg of emodin and 40μg of emodin methyl ether per 1ml.

供试品溶液的制备 Preparation of test solution

取本品粉末（过四号筛）约1g，精密,称定，置具塞锥形瓶中，精密加入甲醇50ml，称定重量，加热回流1小时，取出，放冷，再称定重量，用甲醇补足减失的重量，摇匀，滤过，取续滤液，即得。
Preparation of test solution: Take about 1g of the powder (passed through a No. 4 sieve) of this material, accurately weigh, place it in a stoppered conical flask, accurately add 50ml of methanol, weigh, heat under reflux for 1 hour, take out, cool, weigh again, make up for the lost weight with methanol, shake well, filter, and take the filtrate to obtain the test solution.

测定法 Mensuration

分别精密吸取对照品溶液与供试品溶液各10μl，注入液相色谱仪，测定，即得。
Determination method: Accurately draw 10μl of the reference solution and the test solution, respectively, inject into the liquid chromatograph, and determine to obtain the results.

本品按干燥品计算，含游离蒽醌以大黄素（C15H10O5）和 大黄素甲醚（C16H12O5）的总量计，不得少于0. 10%。
Calculated on the dried basis, the total content of free anthraquinones, represented by emodin (C15H10O5) and emodin methyl ether (C16H12O5), shall not be less than 0.10%.

性 Property

微温。
Slightly warm.

味 Flavor

苦、甘、涩。
Bitter, sweet and astringent.

归经 Meridian tropism

归肝、心、肾经。
Liver, heart, and kidney meridians.

功能 Actions

补肝肾，益精血，乌须发，强筋骨，化浊降脂。
Tonify the liver and kidney, nourish essence and blood, darken hair, strengthen tendons and bones, and resolve turbidity and reduce lipid.

主治 Indications

用于血虚萎黄，眩晕耳鸣，须发早白，腰膝酸软，肢体麻木，崩漏带下，高脂血症。
Used for blood deficiency and withered yellow, dizziness and tinnitus, premature graying of hair, soreness and weakness of the waist and knees, numbness of limbs, excessive uterine bleeding, and hyperlipidemia.

用量 Dosage

6～12g。
6-12g.

用法 Administration

无。
None.

贮藏 Storage

置干燥处，防蛀。
Store in a dry place, protected from moth.

制何首乌

文本参考：《中国药典（2015年版）》
Text reference: Chinese Pharmacopoeia (2015 Edition)

概述 Overview

本品为何首乌的炮制加工品。
The drug is the processed Polygoni Multi-flori Radix.

制法 Procedure

取何首乌片或块，照炖法（通则0213）用黑豆汁拌匀，置非铁质的适宜容器内，炖至汁液吸尽；或照蒸法（通则0213），清蒸或用黑豆汁拌匀后蒸，蒸至内外均呈棕褐色，或晒至半干，切片，干燥。
Procedure Mix the slices or pieces of Polygoni Multiflori Radix thoroughly with black bean juice. Carry out the stewing method <0213> in a suitable non-ferrous container until the juice is exhausted or carry out the steaming method <0213>, steam it alone or steam it after being mixed with black bean juice to a brown colour on all sides, dry in the sun to partial dryness, then cut into slices and dry.

每100kg何首乌片（块），用黑豆10kg。
For each 100 kg of slice (piece) of Polygoni Multiflori Radix use 10 kg of black bean.

黑豆汁制法 取黑豆10kg，加水适量，煮约4小时，熬汁约15kg，豆渣再加水煮约3小时，熬汁约10kg，合并得黑豆汁约25kg。
Preparation of black bean juice: Boil 10 kg of black bean in a sufficient quantity of water for about 4 hours and stew to get about 15 kg of juice. Boil the bean residue again in water for about 3 hours and stew to get about 10 kg of juice. Combine to get about 25 kg of the black bean juice.

性状 Description

本品呈不规则皱缩状的块片，厚约1cm。表面黑褐色或棕褐色，凹凸不平。质坚硬，断面角质样，棕褐色或黑色。气微，味微甘而苦涩。
Irregularly shrunken pieces or slices, about 1 cm thick. Externally dark brown or brown, uneven. Texture hard, fracture horny, brown or black. Odour slight; taste, slightly sweet, bitter and astringent.

鉴别 Identification

照何首乌项下的〔鉴别〕（2）项试验，显相同的结果。
Complies with test (2) of Identification described under Polygoni Multiflori Radix.

检查 Examination

水分 Water

不得过12.0%（通则0832第二法）。
Not more than 12.0 per cent <0832, method 2>.

总灰分 Total ash

不得过9.0% （通则2302）。
Not more than 9.0 per cent <2302>.

浸出物 Extractives

照醇溶性浸出物测定法（通则2201）项下的热浸法测定，用乙醇作溶剂，不得少于5.0%。
Carry out the method for determination of ethanol-soluble extractives <2201, the hot extraction method>, using ethanol as the solvent, not less than 5.0 per cent.

含量测定 Assay

二苯乙烯苷 避光操作。
取本品粉末（过四号筛）约0.2g，精密称定，照何首乌药材﹝含量测定〕项下的方法测定。

游离蔥醌 照高效液相色谱法（通则0512）测定。
色谱条件与系统适用性试验

性味与归经 Property and Flavor

苦、甘、涩，微温。归肝、心、肾经。
Mild warm; bitter, sweet and astringent. Liver, heart and kidney meridians.

功能与主治 Actions

补肝肾，益精血，乌须发，强筋骨，化浊降脂。用于血虚萎黄，眩晕耳鸣，须发早白，腰膝酸软，肢体麻木，崩漏带下，高脂血症。
To tonify the liver-kidney, replenish essence and blood, blacken beard and hair, strengthen sinews and bones, resolve turbidity and lower lipid. Sallow complexion due to blood deficiency, dizziness, tinnitus, premature greying, soreness and weakness in the low back and knees, numbness of the limbs, menstrual flooding and spotting, abnormal vaginal discharge, and hyperlipidemia.

用法与用量 Administration and dosage

6～12g。
6-12 g.

贮藏 Storage

置干燥处，防蛀。
As described under Radix Polygoni Multiflori.