丁公藤

中文文本参考：《中国药典（2020年版）》
English text reference: Chinese Pharmacopoeia (2020 Edition)

概述 Overview

本品为旋花科植物丁公藤Erycibe obtusifolia Benth. 或光叶丁公藤Erycibe schmidtii Craib的干燥藤茎。全年均可采收，切段或片，晒干。
Erycibe Stem is the dried stem of Erycibe obtusifolia Benth. or Erycibe schmidtii Craib (Fam. Convolvulaceae). It can be collected throughout the year, cut into sections or slices, and dried.

性状 Description

本品为斜切的段或片，直径1～10cm。外皮灰黄色、灰褐色或浅棕褐色，稍粗糙，有浅沟槽及不规则纵裂纹或龟裂纹，皮孔点状或疣状，黄白色，老的栓皮呈薄片剥落。质坚硬，纤维较多，不易折断，切面椭圆形，黄褐色或浅黄棕色，异型维管束呈花朵状或块状，木质部导管呈点状。气微，味淡。
The stem is obliquely cut into sections or slices, with a diameter of 1-10 cm. The outer surface is grayish-yellow, grayish-brown, or light brown, slightly rough, with shallow grooves and irregular longitudinal cracks or fissures. The lenticels are dot-shaped or wart-shaped, yellowish-white, and the old cork layer peels off in thin flakes. The texture is hard, with more fibers, not easily broken. The cut surface is elliptical, yellowish-brown or light yellow-brown. The anomalous vascular bundles are flower-like or block-like, and the xylem vessels are dot-shaped. It has a slight odor and a mild taste.

鉴别 Identification

取本品粉末3g，加乙醇40ml，浸渍过夜，加热回流6小时，滤过，滤液加6mol/L盐酸溶液6ml，加热回流3小时，蒸干，残渣加乙醇10ml使溶解，作为供试品溶液。另取东莨菪内酯对照品，加乙醇制成每1ml含0.25mg的溶液，作为对照品溶液。照薄层色谱法（通则0502）试验，吸取上述两种溶液各3μl，分别点于同一硅胶G薄层板上，以环己烷-三氯甲烷-乙酸乙酯-甲酸（6:10:7:1.2）为展开剂，展开，取出，晾干，置紫外光灯（365nm）下检视。供试品色谱中，在与对照品色谱相应的位置上，显相同的亮蓝色荧光斑点。
To 3g of the powder add 40 ml of ethanol, soak overnight, heat under reflux for 6 hours, filter, evaporate the filtrate to dryness, dissolve the residue in 10 ml of ethanol. This is the test solution. Dissolve scopolamine hydrobromide CRS in ethanol to produce a solution containing 0.25 mg per ml as the Reference solution. Carry out the method for thin layer chromatography<0502>, using silica gel G as the coating substance and a mixture of n-hexane, chloroform, ethyl acetate, and formic acid (6:10:7:1.2) as the mobile phase. Apply separately to the plate 3 μl of each of the above two solutions. After developing and removal of the plate, dry in air. Examine under ultraviolet light at 365 nm. The bright blue fluorescent spot in the chromatogram obtained with the test solution corresponds in position and colour to the spot in the chromatogram obtained with the Reference solution.

检查 Examination

水分 Water

不得过12.0%（通则0832第二法）。
Not more than 12.0 percent <0832, method 2>.

总灰分 Total ash

不得过10.0%（通则2302）。
Not more than 10.0 percent <2302>.

浸出物 Extractives

照醇溶性浸出物测定法（通则2201）项下的热浸法测定，用乙醇作溶剂，不得少于3.0%。
Carry out the method for determination of ethanol-soluble Extractives <2201, the hot maceration method>, using ethanol as the solvent, not less than 3.0 percent.

含量测定 Content determination

照高效液相色谱法（通则0512）测定。
Carry out the method for determination of content by high performance liquid chromatography <0512>.

色谱条件与系统适用性试验 Chromatographic conditions and system suitability test

以十八烷基硅烷键合硅胶为填充剂；以甲醇-水-冰醋酸（32∶68∶0.16）为流动相；检测波 长为298nmo理论板数按东莨菪内酯峰计算应不低于2000。
Use octadecylsilane bonded silica gel as the filler; use methanol-water-acetic acid (32:68:0.16) as the mobile phase; the theoretical plate number calculated according to the peak of hyoscyamine should not be less than 2000.

对照品溶液的制备 Preparation of reference solution

取东莨菪内酯对照品适量，精密称定，加甲醇制成每1ml含40μg的溶液，即得。
Take an appropriate amount of hyoscyamine reference substance, accurately weigh, add methanol to make a solution containing 40μg per 1ml.

供试品溶液的制备 Preparation of test solution

取本品粉末（过四号筛）约1g，精密称定，置具塞锥形瓶中，精密加入70%乙醇50ml，称定重量，加热回流6小时，放冷，再称定重量，用70%乙醇补足减失的重量，摇匀，滤过。精密量取续滤液25ml，置烧瓶中，浓缩至约1ml，加3mol/L盐酸溶液10ml，水浴中加热水解2小时， 立即冷却，移入分液漏斗中，用水10ml分次洗涤容器，并入分液漏斗中，加氯化钠2g，用三氯甲烷强力振摇提取5次，每次 15ml，合并三氯甲烷液，加无水硫酸钠2g，搅拌，滤过，容器用少量三氯甲烷洗涤，滤过，滤液合并，70℃以下浓缩至近干， 立即加甲醇使溶解，转移至10ml量瓶中，并稀释至刻度，摇匀，即得。
Take about 1g of the powder of this product (passed through a No. 4 sieve), accurately weigh, place it in a stoppered conical flask, accurately add 50ml of 70% ethanol, weigh, heat under reflux for 6 hours, cool, weigh again, make up for the weight loss with 70% ethanol, shake well, and filter. Accurately take 25ml of the filtrate, place it in a flask, concentrate to about 1ml, add 10ml of 3mol/L hydrochloric acid solution, heat in a water bath for 2 hours, cool immediately, transfer to a separating funnel, wash the container with 10ml of water in portions and add to the separating funnel, add 2g of sodium chloride, extract with chloroform by vigorous shaking for 5 times, each time with 15ml, combine the chloroform solution, add 2g of anhydrous sodium sulfate, stir, filter, wash the container with a small amount of chloroform, and filter. Combine the filtrate, concentrate below 70°C to near dryness, immediately dissolve with methanol, transfer to a 10ml volumetric flask, and dilute to the mark, shake well, and obtain the test solution.

测定法 Determination method

分别精密吸取对照品溶液与供试品溶液各 10μl，注入液相色谱仪，测定，即得。
Accurately take 10μl of the reference solution and the test solution respectively, inject into the liquid chromatograph, and determine.

本品按干燥品计算，含东莨菪内酯（C10H8O4）不得少于 0.050%。
Calculated on the dried product, the content of hyoscyamine (C10H8O4) should not be less than 0.050%.

饮片 Prepared slices

丁公藤 Erycibe Stem

炮制 Processing

除去杂质，洗净，润透，切片，干燥。
Remove impurities, wash, soften, cut into slices, and dry.

性状 Description

本品为椭圆形、长椭圆形或不规则的斜切片，直径1-10cm, 厚0. 2-0. 7cm 。外皮灰黄色、灰褐色或浅棕褐色，有浅纵沟槽，皮孔点状或抚状，黄白色或灰褐色。质坚硬，纤维较多。切面黄褐色或浅黄棕色，异形维管束呈花朵状或块状，木质部导管呈点状。气微，味淡。
This product is oval, long oval or irregular oblique sections, diameter of 1-10cm, thickness of 0.2-0.7cm. The skin is grayish yellow, grayish brown or light brown, with shallow longitudinal grooves, punctate or fusiform pores, yellowish white or grayish brown. Hard, more fiber. The section is yellowish brown or light yellowish brown, the heteromorphic vascular bundles are flower-like or block-like, and the xylem vessels are dot-like. Micro gas, taste light.

鉴别 Identification

同药材。
The same medicinal material.

检查 Examination

同药材。
The same medicinal material.

浸出物 Extractives

同药材。
The same medicinal material.

含量测定 Content determination

同药材。
The same medicinal material.

性 Property

温。
Warm.

毒性 Toxicity

有小毒。
Slightly toxic.

味 Flavor

辛。
Pungent.

归经 Meridian tropism

归肝、脾、胃经。
Liver, spleen, and stomach meridians.

功能 Actions

祛风除湿，消肿止痛。
To dispel wind and dampness, reduce swelling and relieve pain.

主治 Indications

用于风湿痹痛，半身不遂，跌扑肿痛。
Used for rheumatic arthralgia, hemiplegia, and traumatic swelling and pain.

用量 Dosage

3～6g。
3-6g.

用法 Administration

用于配制酒剂，内服或外搽。
Used for preparing wine, taken orally or applied externally.

注意 Precautions

本品有强烈的发汗作用，虚弱者慎用；孕妇禁用。
This product has a strong diaphoretic effect, it should be used with caution in the weak; it is contraindicated in pregnant women.

贮藏 Storage

置干燥处。
Store in a dry place.

丁公藤

文本参考：《中国药典（2015年版）》
Text reference: Chinese Pharmacopoeia (2015 Edition)

概述 Overview

本品为旋花科植物丁公藤Erycibe obtusifolia Benth.或光叶丁公藤Erycibe schmidtii Craib的干燥藤茎。全年均可采收，切段或片，晒干。
Obtuseleaf Erycibe Stem is the dried lianoid stem of Erycibe obtusfolia Benth. or Erycibe schmidtii Craib (Fam. Convolvulaceae). The drug is collected all the year round, cut into section or piece, and dried in the sun.

性状 Description

本品为斜切的段或片，直径1～10cm。外皮灰黄色、灰褐色或浅棕褐色，稍粗糙，有浅沟槽及不规则纵裂纹或龟裂纹，皮孔点状或疣状，黄白色，老的栓皮呈薄片剥落。质坚硬，纤维较多，不易折断，切面椭圆形，黄褐色或浅黄棕色，异型维管束呈花朵状或块状，木质部导管呈点状。气微，味淡。
In oblique sections or pieces, 1-10 cm in diameter. Externally greyish-yellow, greyish-brown or pale brown, slightly rough, with shallow furrows and irregular longitudinal or turtle-like fissures. Lenticles spot-like or protuberant, yellowish-white. Older cork exfoliated. Texture hard, with relatively rich fibres, uneasily broken. Cutsurface elliptical, yellowish-brown or pale yellowish-brown, abnormal vascular bundles flowery or piece-like, vessels of xylem pitted. Odour, slight; taste, weak.

鉴别 Identification

取本品粉末3g，加乙醇40ml，浸渍过夜，加热回流6小时，滤过，滤液加6mol/L盐酸溶液6ml，加热回流3小时，蒸干，残渣加乙醇10ml使溶解，作为供试品溶液。另取东莨菪内酯对照品，加乙醇制成每1ml含0.25mg的溶液，作为对照品溶液。照薄层色谱法（通则0502）试验，吸取上述两种溶液各3μl，分别点于同一硅胶G薄层板上，以环己烷-三氯甲烷-乙酸乙酯-甲酸（6：10：7：1.2）为展开剂，展开，取出，晾干，置紫外光灯（365nm）下检视。供试品色谱中，在与对照品色谱相应的位置上，显相同的亮蓝色荧光斑点。
To 3 g of the powder add 40 ml of ethanol, allow to macerate overnight, heat under reflux for 6 hours, filter. To the filtrate, add 6 ml of hydrochloric acid (6 mol/L), heat under reflux for 3 hours, evaporate to dryness, dissolve the residue in 10 ml of ethanol as the test solution. Dissolve scopoletin CRS in ethanol to produce a solution containing 0.25 mg per ml as the reference solution. Carry out the method for thin layer chromatography<0502> using silica gel G mixed with sodium carboxymethylcellulose as the coating substance and a mixture of cyclohexane, chloroform, ethyl acetate and formic acid (6 : 10 : 7 : 1.2) as the mobile phase. Apply separately to the plate 3 μl of each of the above two solutions. After developing and removal of the plate, dry in air, examine under ultraviolet light at 365 nm. A bright blue fluorescent spot in the chromatogram obtained with the test solution corresponds in position and colour to the spot in the chromatogram obtained with the reference solution.

检查 Examination

水分 Water

不得过12.0%（通则0832第二法）。
Not more than 12.0 per cent <0832, method 2>.

总灰分 Total ash

不得过10.0%（通则2302）。
Not more than 10.0 per cent <2302>.

浸出物 Extractives

照醇溶性浸出物测定法（通则2201）项下的热浸法测定，用乙醇作溶剂，不得少于3.0%。
Carry out the method for determination of ethanol-soluble extractives <2201, the hot extraction method>, using ethanol as the solvent, not less than 3.0 per cent.

含量测定 Assay

照高效液相色谱法（通则0512）测定。
Carry out the method for high performance liquid chromatography <0512>.

色谱条件与系统适用性试验以十八烷基硅烷键合硅胶为填充剂；以甲醇-水-冰醋酸（32：68：0.16）为流动相；检测波长为298nm。理论板数按东莨菪内酯峰计算应不低于2000。
Chromatographic system and system suitability: Use octa-decyl silane bonded silica gel as the stationary phase and a mixture of methanol, water and glacial acetic acid (32 : 68 : 0.16) as the mobile phase. As detector a spectro-photometer set at 298 nm. The number of theoretical plates of the column is not less than 2000, calculated with reference to the peak of scopoletin.

对照品溶液的制备取东莨菪内酯对照品适量，精密称定，加甲醇制成每1ml含40μg的溶液，即得。
Reference solution: Dissolve a quantity of scopoletin CRS, accurately weighed, in methanol to produce a solution containing 40 μg per ml.

供试品溶液的制备取本品粉末（过四号筛）约1g，精密称定，置具塞锥形瓶中，精密加入70%乙醇50ml，称定重量，加热回流6小时，放冷，再称定重量，用70%乙醇补足减失的重量，摇匀，滤过。精密量取续滤液25ml，置烧瓶中，浓缩至约1ml，加3mol/L盐酸溶液10ml，水浴中加热水解2小时，立即冷却，移入分液漏斗中，用水10ml分次洗涤容器，并入分液漏斗中，加氯化钠2g，用三氯甲烷强力振摇提取5次，每次15ml，合并三氯甲烷液，加无水硫酸钠2g，搅拌，滤过，容器用少量三氯甲烷洗涤，滤过，滤液合并，70℃以下浓缩至近干，立即加甲醇使溶解，转移至10ml量瓶中，并稀释至刻度，摇匀，即得。
Test solution: Weigh accurately 1 g of the powder (through No. 4 sieve) to a stopper conical flask. Add accurately 50 ml of 70% ethanol, weigh, heat on a water bath for 6 hours, allow to cool, weigh again, replenish the loss of the weight with 70% ethanol, mix well and filter. Measure accurately 25 ml of the successive filtrate to a flask, concentrate to about 1 ml, add 10 ml of 3 mol/L hydrochloric acid, heat on a water bath for 2 hours, cool immediately. Transfer to a separating funnel, wash the flask several times with 10 ml of water, combine the washings to the separating funnel, add 2 g of sodium chloride, extract with five 15-ml quantities of chloroform, combine the chloroform extracts, add 2 g of anhydrous sodium sulfate, stir well and filter, wash the separating funnel with a small quantity of chloroform, filter, combine the filtrates and evaporate to almost dryness below 70℃ , immediately dissolve the residue in methanol, transfer to a 10 ml volumetric flask, dilute to volume and mix well.

测定法分别精密吸取对照品溶液与供试品溶液各10μl，注入液相色谱仪，测定，即得。
Procedure: Inject accurately 10 μl of each of the reference solution and the test solution, respectively, into the column, and calculate the content.

本品按干燥品计算，含东莨菪内酯（C10H8O4）不得少于0.050%。
It contains not less than 0.050 per cent of scopoletin (CHO), calculated with reference to the dried drug.

饮片 Prepared slices

丁公藤 Obtuseleaf Erycibe Stem

炮制 Processing

除去杂质，洗净，润透，切片，干燥。
Eliminate foreign matter, wash clean, soften thoroughly, cut into slices, and dry in the sun.

性 Property

辛，温；有小毒。
Warm; pungent; slightly toxic.

味 Flavor

辛。
Pungent.

归经 Meridian tropism

归肝、脾、胃经。
Liver, spleen and stomach meridians.

功能 Actions

祛风除湿，消肿止痛。
To dispel wind, remove dampness, resolve swelling and relieve pain.

主治 Indications

用于风湿痹痛，半身不遂，跌扑肿痛。
Painful bi disorder caused by wind-dampness, hemiplegia, swelling and pain caused by traumatic injuries.

用量 Dosage

3～6g，用于配制酒剂，内服或外搽。
3-6 g, used in wine or liquor preparation for oral administration or for topical application.

注意 Precautions and Warnings

本品有强烈的发汗作用，虚弱者慎用；孕妇禁用。
This is a drastic diaphoretic medicine and should be used with caution in persons with weak constitation. Contraindicated for pregnant woman.

贮藏 Storage

置干燥处。
Preserve in a dry place.