莲子心

中文文本参考：《中国药典（2020年版）》
English text reference: Chinese Pharmacopoeia (2020 Edition)

概述 Overview

本品为睡莲科植物莲Nelumbo nucifera Gaertn.的成熟种子中的干燥幼叶及胚根。取出，晒干。
Lotus Plumule is the dried young leaf and embryonic root of Nelumbo nucifera Gaertn. (Fam. Nymphaeaceae). It is collected, removed, and dried.

性状 Description

本品略呈细圆柱形，长1～1.4cm，直径约0.2cm。幼叶绿色，一长一短，卷成箭形，先端向下反折，两幼叶间可见细小胚芽。胚根圆柱形，长约3mm，黄白色。质脆，易折断，断面有数个小孔。气微，味苦。
The drug is slightly cylindrical, measuring 1-1.4 cm long and about 0.2 cm in diameter. The young leaves are green, with one long and one short, rolled into an arrow shape, with the tip folded downward, and a small embryo bud can be seen between the two young leaves. The embryonic root is cylindrical, about 3 mm long, and yellowish-white. The texture is brittle, easily broken, and the fracture surface has several small holes. It has a slight odor and a bitter taste.

鉴别 Identification

（1）本品粉末灰绿色。表皮细胞略呈长方形，壁薄。叶肉细胞壁薄，类圆形，细胞内含众多淀粉粒与绿色色素。胚根细胞呈长方形，排列整齐，壁菲薄，有的含脂肪油滴。幼叶组织中细胞间隙较大。
(1)The powder of the drug is grayish-green. The epidermal cells are slightly rectangular with thin walls. The parenchymal cells have thin walls and are round in shape, containing numerous starch granules and green pigments. The embryonic root cells are rectangular and arranged neatly, with thin walls, and some contain fat droplets. The cell gaps in the young leaf tissue are relatively large.

（2）取本品粉末2g，加甲醇30ml，超声处理30分钟，滤过，滤液蒸干，残渣加甲醇1ml使溶解，作为供试品溶液。另取莲心碱高氯酸盐对照品，加甲醇制成每1ml含1mg的溶液，作为对照品溶液。照薄层色谱法（通则0502）试验，吸取供试品溶液4～6μl、对照品溶液4μl，分别点于同一硅胶G薄层板上，以三氯甲烷-乙酸乙酯-二乙胺（5∶4∶1）为展开剂，展开，取出，晾干，喷以稀碘化铋钾试液。供试品色谱中，在与对照品色谱相应的位置上，显相同颜色的斑点。
(2)To 2 g of the powder add 30 ml of methanol, treat with ultrasound for 30 minutes, filter, evaporate the filtrate to dryness, dissolve the residue in 1 ml of methanol as the test solution. Take the high chlorate salt of lianxin alkaloid as the reference substance, and prepare a solution containing 1 mg per ml in methanol as the reference solution. Carry out the method for thin layer chromatography<0502>, using silica gel G as the coating substance and a mixture of chloroform-acetic ether-diethylamine (5:4:1) as the mobile phase. Apply 4-6 μl of the test solution and 4 μl of the reference solution separately to the same silica gel G thin layer plate. Develop, remove, dry, and spray with a solution of iodine bismuth potassium. The chromatogram obtained with the test solution shows spots of the same color at the corresponding positions as those obtained with the reference solution.

检查 Examination

水分 Water

不得过12.0%（通则0832第二法）。
Not more than 12.0 per cent <0832,method 2>.

总灰分 Total ash

不得过5.0%（通则2302）。
Not more than 5.0 per cent <2302>.

含量测定 Content determination

照高效液相色谱法（通则0512）测定。
Carry out the method for high performance liquid chromatography<0512>.

色谱条件与系统适用性试验 Chromatographic conditions and system suitability test

以十八烷基硅烷键合硅胶为填充剂；以乙腈-0.015mol/L醋酸钠溶液（每100ml中加十二烷基磺酸钠0.4g，再以冰醋酸调pH值至3.0）（52∶48）为流动相；检测波长为282nm。理论板数按甲基莲心碱峰计算应不低于5000。
Use octadecylsilane-bonded silica gel as the filler; use acetonitrile-0.015 mol/L sodium acetate solution (add 0.4 g of dodecyl sodium sulfate to each 100 ml, then adjust the pH value to 3.0 with glacial acetic acid) (52:48) as the mobile phase; the detection wavelength is 282 nm. The theoretical plate number calculated from the peak of methyl lianxin alkaloid should not be less than 5000.

对照品溶液的制备 Preparation of reference solution

取甲基莲心碱对照品适量，精密称定，加甲醇制成每1ml含0.1mg的溶液，即得。
Take an appropriate amount of the reference substance of methyl lianxin alkaloid, accurately weigh, and prepare a solution containing 0.1 mg per ml in methanol.

供试品溶液的制备 Preparation of test solution

取本品粉末（过四号筛）约0.5g，精密称定，精密加入2%盐酸甲醇溶液25ml，称定重量，超声处理（功率250W，频率40kHz）30分钟，放冷，再称定重量，用2%盐酸甲醇溶液补足减失的重量，摇匀，滤过，精密量取续滤液5ml，蒸至近干，残渣用甲醇溶解，转移至10ml量瓶中，并稀释至刻度，摇匀，滤过，取续滤液，即得。
Take about 0.5 g of the powder of the drug (passed through a No. 4 sieve), accurately weigh, accurately add 25 ml of 2% hydrochloric acid methanol solution, weigh, treat with ultrasound (power 250 W, frequency 40 kHz) for 30 minutes, cool, weigh again, make up for the lost weight with 2% hydrochloric acid methanol solution, shake well, filter, accurately take 5 ml of the subsequent filtrate, evaporate to near dryness, dissolve the residue in methanol, transfer to a 10 ml volumetric flask, dilute to the mark, shake well, filter, and take the subsequent filtrate.

测定法 Assay method

分别精密吸取对照品溶液与供试品溶液各10μl，注入液相色谱仪，测定，即得。
Accurately take 10 μl of the reference solution and the test solution separately, inject into the liquid chromatograph, and determine.

本品按干燥品计算，含甲基莲心碱（C38H45N2O6）不得少于0.70%。
Calculated on the dried basis, the content of methyl lianxin alkaloid (C38H45N2O6) should not be less than 0.70%.

性 Property

寒。
Cold.

味 Flavor

苦。
Bitter.

归经 Meridian tropism

归心、肾经。
Heart and kidney meridians

功能 Actions

清心安神，交通心肾，涩精止血。
To clear the heart and calm the mind, communicate the heart and kidney, astringe essence and stop bleeding.

主治 Indications

用于热入心包，神昏谵语，心肾不交，失眠遗精，血热吐血。
Used for heat entering the pericardium, coma and delirium, disharmony between the heart and kidney, insomnia and nocturnal emission, and hematemesis caused by blood heat.

用量 Dosage

2 ～5g。
2-5 g.

用法 Administration

无。
None.

贮藏 Storage

置通风干燥处，防潮，防蛀。
Store in a well-ventilated, dry place, protected from moisture and insects.

莲子心

文本参考：《中国药典（2015年版）》
Text reference: Chinese Pharmacopoeia (2015 Edition)

概述 Overview

本品为睡莲科植物莲 Nelumbo nucifera Gaertn. 的成熟种子中的干燥幼叶及胚根。取出，晒干。
Lotus Plumule is the dried young cotyledon and radicle of the ripe seed of Nelumbo nucifera Gaertrn. (Fam. Nymphaeaceae). The drug is taken out of the seed and dried in the sun.

性状 Description

本品略呈细圆柱形，长1～1.4cm，直径约0.2cm。幼叶绿色，一长一短，卷成箭形，先端向下反折，两幼叶间可见细小胚芽。胚根圆柱形，长约3mm，黄白色。质脆，易折断，断面有数个小孔。气微，味苦。
Fine-cylindrical, 1-1.4 cm long, about 0.2 cm in diameter. The young cotyledons green, one long and the other one short, curved to arrow-shaped, apex folded downwards, a fine plumule visible between the two cotyledons. Radicle cylindrical, about 3 mm long, yellowish-white. Texture fragile, easily broken, fracture showing several small pits. Odour, slight; taste, bitter.

鉴别 Identification

（1）本品粉末灰绿色。表皮细胞略呈长方形，壁薄。叶肉细胞壁薄，类圆形，细胞内含众多淀粉粒与绿色色素。胚根细胞呈长方形，排列整齐，壁菲薄，有的含脂肪油滴。幼叶组织中细胞间隙较大。
（2）取本品粉末2g，加甲醇30ml，超声处理30分钟，滤过，滤液蒸干，残渣加甲醇1ml使溶解，作为供试品溶液。另取莲心碱高氯酸盐对照品，加甲醇制成每1ml含1mg的溶液，作为对照品溶液。照薄层色谱法（通则0502）试验，吸取供试品溶液4～6μl、对照品溶液化4μl，分别点于同一硅胶G薄层板上，以三氯甲烷-乙酸乙酯-二乙胺（5：4：1）为展开剂，展开，取出，晾干，喷以稀碘化铋钾试液。供试品色谱中，在与对照品色谱相应的位置上，显相同颜色的斑点。

检查 Examination

水分 Water

不得过12.0%（通则0832第二法）。
Not more than 12.0 per cent <0832, method 2>.

总灰分 Total ash

不得过5.0%（通则2302）。
Not more than 5.0 per cent <2302>.

含量测定 Assay

照高效液相色谱法（通则0512）测定。
Carry out the method for high performance liquid chromatography <0512>.

色谱条件与系统适用性试验 Chromatographic system and system suitability

以十八烷基硅烷键合硅胶为填充剂；以乙腈-0.015mol/L十二烷基磺酸钠溶液（取十二烷基磺酸钠0.41g，加水100ml使溶解）-冰醋酸（56：43：1）为流动相；检测波长为282nm。理论板数按莲心碱峰计算应不低于5000。
Use octadecylsilane bonded silica gel as the stationary phase and a mixture of acetonitrile, 0.015 mol/L of dodecyl sodium sulfonate (dissolve 0.41 g of dodecyl sodium sulfonate in 100 ml water) and glacial acetic acid (56 : 43 : 1) as the mobile phase. As detector a spectrophotometer set at 282 nm. The number of theoretical plates of the column is not less than 5000, calculated with the reference to the peak of limensinine.

对照品溶液的制备 Reference solution

取莲心碱高氯酸盐对照品适量，精密称定，加甲醇制成每1ml含25μg的溶液，即得（莲心碱重量=莲心碱高氯酸盐重量/1.3587）。
Weigh accurately a quantity of liensinine perchlorate CRS, add methanol to prepare a solution containing 25 μg per ml. (weight of liensinine＝weight of liensinine perchlorate/1.3587).

供试品溶液的制备 Test solution

取本品粉末（过四号筛）约0.5g，精密称定，精密加入2%盐酸甲醇溶液25ml，称定重量，加热回流30分钟，放冷，再称定重量，用2%盐酸甲醇溶液补足减失的重量，摇匀，滤过，精密量取续滤液5ml，置10ml量瓶中，加流动相稀释至刻度，摇匀，即得。
Weigh accurately about 0.5 g of the powder (through No. 4 sieve) in a flask, add accurately 25 ml of 2% hydrochloric acid in methanol, weigh, heat under reflux for 30 minutes, allow to cool, weigh again and replenish the loss of solvent with 2% hydrochloric acid in methanol, filter, measure accurately 5 ml of the successive filtrate to a 10 ml volumetric flask, add mobile phase to volume, mix well as the test solution.

测定法 Procedure

分别精密吸取对照品溶液与供试品溶液各10μl，注入液相色谱仪，测定，即得。
Inject accurately 10 μl of each of the reference solution and the test solution, respectively, into the column, determine and calculate the content.

本品按干燥品计算，含莲心碱（C37H42N2O6）不得少于0.20%。
It contains not less than 0.20 per cent of liensinine (C37H42N2O6) calculated with reference to the dried drug.

性味与归经 Property and Flavor

苦，寒。归心、肾经。
Cold; bitter.

功能与主治 Actions and Indications

清心安神，交通心肾，涩精止血。用于热入心包，神昏谵语，心肾不交，失眠遗精，血热吐血。
To clear the heart, tranquilize the mind, coordinate the heart and kidney, astringe essence and stanch bleeding. Indicated for heat entering the pericardium, loss of consciousness, delirious speech, heart-kidney non-interaction pattern, insomnia, seminal emission, and hematemesis caused by blood heat.

用法与用量 Administration and dosage

2～5g。
2-5 g.

贮藏 Storage

置通风干燥处，防潮，防蛀。
Preserve in a ventilated and dry place, and protect from mould and moth.