路路通

中文文本参考：《中国药典（2020年版）》
English text reference: Chinese Pharmacopoeia (2020 Edition)

概述 Overview

本品为金缕梅科植物枫香树Liquidambar formosana Hance的干燥成熟果序。冬季果实成熟后采收，除去杂质，干燥。
Liquidambar Fruit is the dried mature fruit cluster of Liquidambar formosana Hance (Fam. Hamamelidaceae). The drug is collected in winter when the fruit is mature, removed from impurities, and dried.

性状 Description

本品为聚花果，由多数小蒴果集合而成，呈球形，直径2～3cm。基部有总果梗。表面灰棕色或棕褐色，有多数尖刺和喙状小钝刺，长0.5～1mm，常折断，小蒴果顶部开裂，呈蜂窝状小孔。体轻，质硬，不易破开。气微，味淡。
The fruit cluster is polymeric, formed by numerous small follicles, spherical, 2-3 cm in diameter. The base has a common peduncle. The surface is gray-brown or brownish-brown, with numerous sharp spines and beak-like small blunt spines, 0.5-1 mm long, often broken, and the top of the small follicle cracks, forming a honeycomb-like small hole. The body is light, hard in texture, and not easily broken. The odor is slight and the taste is mild.

鉴别 Identification

（1）本品粉末棕褐色。纤维多碎断，直径13～45μm，末端稍钝或钝圆，壁多波状弯曲，木化，胞腔宽或窄，内常含棕黄色物。果皮石细胞类方形、棱形、不规则形或分枝状，直径53～398μm，壁极厚，孔沟分枝状。表皮细胞断面观长方形，长34～55μm；表面观多角形，直径6～17μm，壁厚，具孔沟，内含棕黄色物。单细胞非腺毛，常弯曲，长42～126μm，基部宽11～19μm，含棕黄色物。
(1) The powder of this product is brownish-brown. The fibers are mostly fragmented, with a diameter of 13-45μm, slightly blunt or blunt at the end, and the wall is mostly wavy and curved, lignified, with wide or narrow cell cavities, often containing brownish-yellow substances. The stone cells of the fruit peel are square, angular, irregular, or branched, with a diameter of 53-398μm, and the wall is extremely thick, with branched pores. The epidermal cells have a rectangular cross-section, with a length of 34-55μm; the surface view is polygonal, with a diameter of 6-17μm, thick wall, with pores, and containing brownish-yellow substances. The single-cell non-glandular hairs are often curved, with a length of 42-126μm and a width of 11-19μm at the base, containing brownish-yellow substances.

（2）取本品粉末2g，加乙酸乙酯50ml，超声处理30分钟，滤过，滤液置水浴上浓缩至约2ml，加于中性氧化铝柱（200～300目，2g，内径为10mm）上，用乙酸乙酯25ml洗脱，弃去洗脱液，再以50%甲醇25ml洗脱，收集洗脱液，蒸干，残渣加乙酸乙酯1ml使溶解，作为供试品溶液。另取路路通酸对照品，加乙酸乙酯制成每1ml含1mg的溶液，作为对照品溶液。照薄层色谱法（通则0502）试验，吸取上述两种溶液各6μl，分别点于同一硅胶G薄层板上，使成条状，以甲苯-乙酸乙酯-甲酸（20∶2∶1）5～10℃放置12小时的上层溶液为展开剂，展开缸预平衡15分钟，展开，取出，晾干，喷以1%香草醛的10%硫酸乙醇溶液，80℃加热至斑点显色清晰。供试品色谱中，在与对照品色谱相应的位置上，显相同颜色的条斑。
(2) Take 2g of the powder, add 50ml of ethyl acetate, and sonicate for 30 minutes. Filter and concentrate the filtrate on a water bath to about 2ml. Add it to a neutral alumina column (200-300 mesh, 2g, inner diameter 10mm), elute with 25ml of ethyl acetate, discard the eluate, and elute again with 50% methanol 25ml. Collect the eluate, evaporate to dryness, dissolve the residue in 1ml of ethyl acetate as the test solution. Take Lulutong acid reference substance, add ethyl acetate to make a solution containing 1mg per ml as the reference solution. Carry out the method for thin layer chromatography<0502>, using silica gel G as the coating substance and a mixture of toluene-ethyl acetate-formic acid (20:2:1) as the mobile phase. Apply separately to the plate 6 μl of each of the above two solutions. After developing and removal of the plate, dry in air. Spray with a 1% solution of vanillin in 10% sulfuric acid in ethanol, heat at 80°C to the spots clear. The chromatographic spot in the test solution corresponds in position and colour to the spot in the chromatogram obtained with the reference solution.

检查 Examination

水分 Water

不得过9.0%（通则0832第二法）。
Not more than 9.0 per cent <0832,method 2>.

总灰分 Total ash

不得过5.0%（通则2302）。
Not more than 5.0 per cent <2302>.

酸不溶性灰分 Acid-insoluble ash

不得过2.5%（通则2302）。
Not more than 2.5 per cent <2302>.

含量测定 Assay

照高效液相色谱法（通则0512）测定。
Determine by high-performance liquid chromatography <0512>.

色谱条件与系统适用性试验 Chromatographic conditions and system suitability test

以十八烷基硅烷键合硅胶为填充剂；以甲醇-水-冰醋酸（87∶13∶0.1）为流动相；蒸发光散射检测器检测。理论板数按路路通酸峰计算应不低于6000。
Using octadecylsilane bonded silica gel as the filler; methanol-water-glacial acetic acid (87:13:0.1) as the mobile phase; detected by evaporative light scattering detector. The theoretical plate number of the peak of rutaecarpine should not be less than 6000.

对照品溶液的制备 Preparation of reference solution

取路路通酸对照品适量，精密称定，置棕色量瓶中，加无水乙醇制成每1ml含0.3mg的溶液，即得。
Take an appropriate amount of rutaecarpine reference substance, accurately weigh it, place it in a brown volumetric flask, add anhydrous ethanol to make a solution containing 0.3 mg per 1 ml.

供试品溶液的制备 Preparation of test solution

取本品粉末（过三号筛）约0.6g，精密称定，置具塞锥形瓶中，精密加入无水乙醇20ml，称定重量，超声处理15分钟，放冷，再称定重量，用无水乙醇补足减失的重量，摇匀，滤过，精密量取续滤液10ml，蒸干，残渣加无水乙醇溶解，转移至2ml量瓶中，加无水乙醇至刻度，摇匀，滤过，取续滤液，即得。
Take about 0.6g of the powder of this product (passed through a No. 3 sieve), accurately weigh it, place it in a stoppered conical flask, accurately add 20ml of anhydrous ethanol, weigh it, treat it with ultrasound for 15 minutes, let it cool, weigh it again, make up for the lost weight with anhydrous ethanol, shake well, filter, accurately take 10ml of the filtrate, evaporate to dryness, dissolve the residue with anhydrous ethanol, transfer it to a 2ml volumetric flask, add anhydrous ethanol to the mark, shake well, filter, and take the filtrate.

测定法 Assay method

分别精密吸取对照品溶液5μl、8μl，供试品溶液5μl，注入液相色谱仪，测定，用外标两点法对数方程计算，即得。
Accurately take 5μl and 8μl of the reference solution, and 5μl of the test solution, inject into the liquid chromatograph, determine, calculate by the logarithmic equation of the external standard two-point method, and obtain the result.

本品按干燥品计算，含路路通酸（C30H46O3）不得少于0.15%。
Calculated on the dried product, the content of rutaecarpine (C30H46O3) should not be less than 0.15%.

性 Property

平。
Neutral.

味 Flavor

苦。
Bitter.

归经 Meridian tropism

归肝、肾经。
Liver and kidney meridians.

功能 Actions

祛风活络，利水，通经。
Expel wind and activate collaterals, promote diuresis, and regulate menstruation.

主治 Indications

用于关节痹痛，麻木拘挛，水肿胀满，乳少，经闭。
Used for joint pain, numbness and spasm, edema and fullness, insufficient lactation, and amenorrhea.

用量 Dosage

5～10g。
5～10g.

用法 Administration

无。
None.

贮藏 Storage

置干燥处。
Store in a dry place.

路路通

文本参考：《中国药典（2015年版）》
Text reference: Chinese Pharmacopoeia (2015 Edition)

概述 Overview

本品为金缕梅科植物枫香树Liquidambar formosana Hance的干燥成熟果序。冬季果实成熟后采收，除去杂质，干燥。
Beautiful Sweetgum Fruit is the dried ripe infructescences of Liquidambar formosana Hance (Fam. Hamamelidaceae). The drug is collected in winter when the fruit is ripe, removed from foreign matter, and dried.

性状 Description

本品为聚花果，由多数小蒴果集合而成，呈球形，直径2～3cm。基部有总果梗。表面灰棕色或棕褐色，有多数尖刺和瞭状小钝刺，长0.5～1mm，常折断，小蒴果顶部开裂，呈蜂窝状小孔。体轻，质硬，不易破开。气微，味淡。
Collective fruit, composed of numerous small capsules, spheroidal, 2-3 cm in diameter, with a fruit stalk at the base. Externally greyish-brown or brown, bearing numerous acute spines and small beaked obtuse spines, 0.5-1 mm long, often broken. Small capsules acrocidal, showing small honeycomb-shaped boles. Texture light and hard, uneasily broken. Odour, slight; taste, weak.

鉴别 Identification

（1）本品粉末棕褐色。纤维多碎断，直径13～45μm，末端稍钝或钝圆，壁多波状弯曲，木化，胞腔宽或窄，内常含棕黄色物。果皮石细胞类方形、棱形、不规则形或分枝状，直径53～398μm，壁极厚，孔沟分枝状。表皮细胞断面观长方形，长34～55μm；表面观多角形，直径6～17μm，壁厚，具孔沟，内含棕黄色物。单细胞非腺毛，常弯曲，长42～126μm，基部宽11～19μm，含棕黄色物。
（2）取本品粉末2g，加乙酸乙酯50ml，超声处理30分钟，滤过，滤液置水浴上浓缩至约2ml，加于中性氧化铝柱（200～300目，2g，内径为10mm）上，用乙酸乙酯25ml洗脱，弃去洗脱液，再以50%甲醇25ml洗脱，收集洗脱液，蒸干，残渣加乙酸乙酯1ml使溶解，作为供试品溶液。另取路路通酸对照品，加乙酸乙酯制成每1ml含1mg的溶液，作为对照品溶液。照薄层色谱法（通则0502）试验，吸取上述两种溶液各6μl，分别点于同一硅胶G薄层板上，使成条状，以甲苯-乙酸乙酯-甲酸（20：2：1）5～10℃放置12小时的上层溶液为展开剂，展开缸预平衡15分钟，展开，取出，晾干，喷以1%香草醛的10%硫酸乙醇溶液，80℃加热至斑点显色清晰。供试品色谱中，在与对照品色谱相应的位置上，显相同颜色的条斑。

检查 Examination

水分 Water

不得过9.0%（通则0832第二法）。
Not more than 9.0 per cent <0832, method 2>.

总灰分 Total ash

不得过5.0%（通则2302）。
Not more than 5.0 per cent <2302>.

酸不溶性灰分 Acid-insoluble ash

不得过2.5%（通则2302）。
Not more than 2.5 per cent <2302>.

含量测定 Assay

照高效液相色谱法（通则0512）测定。
Carry out the method for high performance liquid chromatography <0512>.

贮藏 Storage

置干燥处。
Preserve in a dry place.