神农Alpha
天然药材
宁夏枸杞果实
NMM
Natural Medicinal Material
CNMM
Chinese Natural Medicinal Material
中国药典(2020年版)收录条目
中国药典(2015年版)收录条目
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摘要Abstract

根据《中国药典·2020年版·一部》记载:本品为茄科植物宁夏枸杞Lycium barbarum L.的干燥成熟果实。夏、秋二季果实呈红色时采收,热风烘干,除去果梗,或晾至皮皱后,晒干,除去果梗。
This product is the dried mature fruit of the Solanaceae plant Lycium barbarum L., also known as Ningxia wolfberry. The fruit is harvested in summer and autumn when it turns red, then dried with hot air and the fruit stem is removed. Alternatively, it is air-dried until the skin wrinkles, then sun-dried and the fruit stem is removed.


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天然药材系统命名法Systematic Nomenclature for Natural Medicinal Materials
NMM ID
nmm-00tt
系统名
Lycium barbarum Fruit
系统中文名
宁夏枸杞果实(níng xià gǒu qǐ guǒ shí)
通用名
Gou-qi-zi
通用中文名
枸杞子(gǒu qǐ zǐ)
标准化译名
Lycium barbarum Fruit (NMM-00TT, Gou-qi-zi)
标准化中文译名
宁夏枸杞果实(NMM-00TT,枸杞子)
类型
plant
物种基源
Lycium barbarum | 宁夏枸杞
药用部位
fruit | 果实
特殊描述
炮制方法
系统名命名解释

根据《中国药典·2020年版·一部》记载:本品为茄科植物宁夏枸杞Lycium barbarum L.的干燥成熟果实。夏、秋二季果实呈红色时采收,热风烘干,除去果梗,或晾至皮皱后,晒干,除去果梗。
This product is the dried mature fruit of the Solanaceae plant Lycium barbarum L., also known as Ningxia wolfberry. The fruit is harvested in summer and autumn when it turns red, then dried with hot air and the fruit stem is removed. Alternatively, it is air-dried until the skin wrinkles, then sun-dried and the fruit stem is removed.

通用名命名解释

NMMGN衍生自《中国药典·2020年版·一部》相关中药材中文名。
NMMGN follows the Chinese name of the relevant Chinese medicinal materials in the Chinese Pharmacopoeia: 2020 edition: Volume I.

上级药材
下级药材
创建人
审核专家
3
中国药典Chinese Pharmacopoeia
3.1
中国药典(2020年版)Chinese Pharmacopoeia (2020 Edition)

关于
中
En
中国药典(2020年版)是2020年出版的第11版中国药典,其为中国现行的药典标准。中国药典(2020年版)收录中药材品种616种。

枸杞子

中文文本参考:《中国药典(2020年版)》
English text reference: Chinese Pharmacopoeia (2020 Edition)

枸杞子
Gouqizi
Lycii Fructus
Lycium Fruit

概述 Overview

本品为茄科植物宁夏枸杞Lycium barbarum L.的干燥成熟果实。夏、秋二季果实呈红色时采收,热风烘干,除去果梗,或晾至皮皱后,晒干,除去果梗。
Lycium Fruit is the dried ripe fruit of Lycium barbarum L. (Fam. Solanaceae). The fruit is collected in summer and autumn when it is red, dried in hot air, removed from the pedicel, or dried in the sun until the skin is wrinkled and then dried, and the pedicel is removed.

性状 Description

本品呈类纺锤形或椭圆形,长6~20 mm,直径3~10 mm。表面红色或暗红色,顶端有小突起状的花柱痕,基部有白色的果梗痕。果皮柔韧,皱缩;果肉肉质,柔润。种子20~50粒,类肾形,扁而翘,长1.5~1.9 mm,宽1-1.7 mm,表面浅黄色或棕黄色。气微,味甜。
Fruits spindle-shaped or ovoid, 6-20 mm long, 3-10 mm in diameter; surface red or dark red, with a small protuberant style scar at the apex and a white pedicel scar at the base; pericarp tough, wrinkled; flesh fleshy, soft; seeds 20-50, reniform, compressed and curved, 1.5-1.9 mm long, 1-1.7 mm wide, light yellow or brownish-yellow on the surface. Odour, slight; taste, sweet.

鉴别 Identification

(1)本品粉末黄橙色或红棕色。外果皮表皮细胞表面观呈类多角形或长多角形,垂周壁平直或细波状弯曲,外平周壁表面有平行的角质条纹。中果皮薄壁细胞呈类多角形,壁薄,胞腔内含橙红色或红棕色球形颗粒。种皮石细胞表面观不规则多角形,壁厚,波状弯曲,层纹清晰。
(1)The powder is yellow-orange or reddish-brown. The epidermal cells of the outer pericarp are polygonal or elongated polygonal in surface view, with straight or slightly wavy walls, and the outer periclinal walls have parallel cuticular striations. The thin-walled cells of the middle pericarp are polygonal, with thin walls, and contain orange-red or reddish-brown spherical particles in the cell cavity. The surface of the seed coat stone cells is irregularly polygonal, with thick walls and clear layered patterns.

(2)取本品0.5 g,加水35 ml,加热煮沸15分钟,放冷,滤过,滤液用乙酸乙酯15 ml振摇提取,分取乙酸乙酯液,浓缩至1 ml,作为供试品溶液。另取枸杞子对照药材0.5 g,同法制成对照药材溶液。照薄层色谱法(通则0502)试验,吸取上述两种溶液各5 μl,分别点于同一硅胶G薄层板上,以乙酸乙酯-三氯甲烷-甲酸(3∶2∶1)为展开剂,展开,取出,晾干,置紫外光灯(365 nm)下检视。供试品色谱中,在与对照药材色谱相应的位置上,显相同颜色的荧光斑点。
(2)Take 0.5 g of the powder, add 35 ml of water, heat to boiling for 15 minutes, cool, filter, extract the filtrate with 15 ml of ethyl acetate, concentrate the ethyl acetate solution to 1 ml as the test solution. Take 0.5 g of the reference drug of Lycium Fruit, prepare the reference drug solution by the same method. Carry out the method for thin layer chromatography <0502>, using silica gel G as the coating substance and a mixture of ethyl acetate-chloroform-formic acid (3: 2: 1) as the mobile phase. Apply separately to the plate 5 μl of each of the above two solutions. After developing and removal of the plate, dry in air. Examine under ultraviolet light at 365 nm. The fluorescent spot in the chromatogram obtained with the test solution corresponds in position and colour to the spot in the chromatogram obtained with the Reference solution.

检查 Examination

水分 Water

不得过13.0%(通则0832第二法,温度为80 °C)。
Not more than 13.0 per cent <0832, method 2>, at 80 °C.

总灰分 Total ash

不得过5.0%(通则2302)。
Not more than 5.0 per cent <2302>.

重金属及有害元素 Heavy metals and harmful elements

照铅、镉、砷、汞、铜测定法(通则2321原子吸收分光光度法或电感耦合等离子体质谱法)测定,铅不得过5 mg/kg;镉不得过1 mg/kg;砷不得过2 mg/kg;汞不得过0.2 mg/kg;铜不得过20 mg/kg。
Determined by the method for the determination of lead, cadmium, arsenic, mercury, and copper <2321> (atomic absorption spectrophotometry or inductively coupled plasma mass spectrometry). The limit of lead is not more than 5 mg/kg; the limit of cadmium is not more than 1 mg/kg; the limit of arsenic is not more than 2 mg/kg; the limit of mercury is not more than 0.2 mg/kg; the limit of copper is not more than 20 mg/kg.

浸出物 Extractives

照水溶性浸出物测定法(通则2201)项下的热浸法测定,不得少于55.0%。
Determined by the method for determination of water-soluble extractives <2201>, the hot maceration method. Not less than 55.0 per cent.

含量测定 Assay

枸杞多糖 Lycium Polysaccharides

对照品溶液的制备 Preparation of reference solution

取无水葡萄糖对照品25 mg,精密称定,置250 ml量瓶中,加水适量溶解,稀释至刻度,摇匀,即得(每1 ml中含无水葡萄糖0.1 mg)。
Weigh accurately 25 mg of anhydrous glucose reference substance, place it in a 250 ml volumetric flask, dissolve it in a suitable amount of water, dilute to the mark, shake well, and obtain a solution containing 0.1 mg of anhydrous glucose per ml.

标准曲线的制备 Preparation of standard curve

精密量取对照品溶液0.2 ml、0.4 ml、0.6 ml、0.8 ml、1.0 ml,分别置具塞试管中,分别加水补至2.0 ml,各精密加入5%苯酚溶液1 ml,摇匀,迅速精密加入硫酸5 ml,摇匀,放置10分钟,置40 ℃水浴中保温15分钟,取出,迅速冷却至室温,以相应的试剂为空白,照紫外-可见分光光度法(通则0401),在490 nm的波长处测定吸光度,以吸光度为纵坐标,浓度为横坐标,绘制标准曲线。
Accurately measure 0.2 ml, 0.4 ml, 0.6 ml, 0.8 ml, and 1.0 ml of the reference solution, place them in separate stoppered test tubes, add water to each tube to make the volume 2.0 ml, add 1 ml of 5% phenol solution accurately to each tube, shake well, add 5 ml of sulfuric acid accurately to each tube, shake well, let stand for 10 minutes, keep warm in a 40 °C water bath for 15 minutes, take out, cool rapidly to room temperature, use the corresponding reagent as a blank, and determine the absorbance at a wavelength of 490 nm by ultraviolet-visible spectrophotometry <0401>. Plot the standard curve with absorbance as the ordinate and concentration as the abscissa.

测定法 Assay method

取本品粗粉约0.5 g,精密称定,加乙醚100 ml,加热回流1小时,静置,放冷,小心弃去乙醚液,残渣置水浴上挥尽乙醚。加入80%乙醇100 ml,加热回流1小时,趁热滤过,滤渣与滤器用热80%乙醇30 ml分次洗涤,滤渣连同滤纸置烧瓶中,加水150 ml,加热回流2小时。趁热滤过,用少量热水洗涤滤器,合并滤液与洗液,放冷,移至250 ml量瓶中,用水稀释至刻度,摇匀,精密量取1 ml,置具塞试管中,加水1.0 ml,照标准曲线的制备项下的方法,自“各精密加入5%苯酚溶液1 ml”起,依法测定吸光度,从标准曲线上读出供试品溶液中含葡萄糖的重量(mg),计算,即得。
Take about 0.5 g of the powder, accurately weigh it, add 100 ml of ether, heat under reflux for 1 hour, let stand, cool, carefully discard the ether, evaporate the residue on a water bath to remove the ether. Add 100 ml of 80% ethanol, heat under reflux for 1 hour, filter while hot, wash the residue and the filter with 30 ml of hot 80% ethanol in portions, place the residue together with the filter paper in a flask, add 150 ml water, heat under reflux for 2 hours. Filter while hot, wash the filter with a small amount of hot water, combine the filtrate and the washings, cool, transfer to a 250 ml volumetric flask, dilute to the mark with water, shake well, accurately measure 1 ml, place it in a stoppered test tube, add 1.0 ml of water, and determine the absorbance according to the method described in the preparation of the standard curve, starting from "add 1 ml of 5% phenol solution accurately" in the method. Read the weight of glucose (mg) in the test solution from the standard curve and calculate.

本品按干燥品计算,含枸杞多糖以葡萄糖(C6H12O6)计,不得少于1.8%。
Calculated on the dried basis, the content of Lycium Polysaccharides, calculated as glucose (C6H12O6), should not be less than 1.8%.

甜菜碱 Betaine

照高效液相色谱法(通则0512)测定。
Determined by the method for determination of betaine by high performance liquid chromatography <0512>.

色谱条件与系统适用性试验 Chromatographic conditions and system suitability test

以氨基键合硅胶为填充剂;以乙腈-水(85∶15)为流动相;检测波长为195 nm。理论板数按甜菜碱峰计算应不低于3000。
Using aminopropyl-bonded silica gel as the filler; using acetonitrile-water (85: 15) as the mobile phase; the detection wavelength is 195 nm. The theoretical number of plates calculated from the peak of betaine should not be less than 3000.

对照品溶液的制备 Preparation of reference solution

取甜菜碱对照品适量,精密称定,加水制成每1 ml含0.17 mg的溶液,即得。
Take an appropriate amount of betaine reference substance, accurately weigh it, dissolve it in water to make a solution containing 0.17 mg per ml.

供试品溶液的制备 Preparation of test solution

取本品粉碎,取约1 g,精密称定,置具塞锥形瓶中,精密加入甲醇50 ml,密塞,称定重量,加热回流1小时,放冷,再称定重量,用甲醇补足减失的重量,摇匀,滤过。精密量取续滤液2 ml,置碱性氧化铝固相萃取柱(2 g)上,用乙醇30 ml洗脱,收集洗脱液,蒸干,残渣加水溶解,转移至2 ml量瓶中,加水至刻度,摇匀,滤过,取续滤液,即得。
Take about 1 g of the powder, accurately weigh it, place it in a stoppered conical flask, accurately add 50 ml of methanol, seal the flask, weigh it, heat under reflux for 1 hour, cool, weigh it again, make up for the weight loss with methanol, shake well, filter. Accurately measure 2 ml of the subsequent filtrate, place it on an alkaline alumina solid phase extraction column (2 g), elute with 30 ml of ethanol, collect the eluate, evaporate to dryness, dissolve the residue in water, transfer to a 2 ml volumetric flask, dilute to the mark with water, shake well, filter, and take the subsequent filtrate.

测定法 Assay method

分别精密吸取对照品溶液与供试品溶液各10 μl,注入液相色谱仪,测定,即得。
Accurately aspirate 10 μl of the reference solution and the test solution, respectively, into the liquid chromatograph, and determine.

本品按干燥品计算,含甜菜碱(C5H11NO2)不得少于0.50%。
Calculated on the dried basis, the content of betaine (C5H11NO2) should not be less than 0.50%.

性 Property

平。
Neutral.

味 Flavor

甘。
Sweet.

归经 Meridian tropism

归肝、肾经。
Liver and kidney meridians.

功能 Actions

滋补肝肾,益精明目。
Nourish the liver and kidney, nourish essence, and improve vision.

主治 Indications

用于虚劳精亏,腰膝酸痛,眩晕耳鸣,阳萎遗精,内热消渴,血虚萎黄,目昏不明。
Used for deficiency of essence and fatigue, soreness of the waist and knees, dizziness and tinnitus, impotence and premature ejaculation, internal heat and polydipsia, blood deficiency and withered yellow, and blurred vision.

用量 Dosage

6~12 g。
6-12 g.

用法 | Administration

无。
None.

贮藏 Storage

置阴凉干燥处,防闷热,防潮,防蛀。
Preserve in a cool and dry place, protected from heat, moisture, and moth.


创建人:
创建于:
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3.2
中国药典(2015年版)Chinese Pharmacopoeia (2015 Edition)

关于
中
En
中国药典(2015年版)是2015年出版的第10版中国药典。中国药典(2015年版)收录中药材品种618种。

枸杞子

中文文本参考:《中国药典(2015年版)》
English text reference: Chinese Pharmacopoeia (2015 Edition)

枸杞子
Gouqizi
Lycii Fructus
Lycium Fruit

概述 Overview

本品为茄科植物宁夏枸杞 Lycium barbarum L. 的干燥成熟果实。夏、秋二季果实呈红色时采收,热风烘干,除去果梗,或晾至皮皱后,晒干,除去果梗。
Lycium Fruit is the dried ripe fruit of Lycium barbarum L. (Fam. Solanaceae). The fruit is collected in summer and autumn when it turns red, dried by hot air and the pedicel removed; or it is air-dried until the skin becomes wrinkled, then sun-dried and the pedicel removed.

性状 Description

本品呈类纺锤形或椭圆形,长 6~20 mm,直径 3~10 mm。表面红色或暗红色,顶端有小突起状的花柱痕,基部有白色的果梗痕。果皮柔韧,皱缩;果肉肉质,柔润。种子 20~50 粒,类肾形,扁而翘,长 1.5~1.9 mm,宽 1~1.7 mm,表面浅黄色或棕黄色。气微,味甜。
Fruits are spindle-shaped or ovoid, 6–20 mm long and 3–10 mm in diameter. The surface is red or dark red, with a small protuberant style scar at the apex and a white pedicel scar at the base. The pericarp is tough and wrinkled; the pulp is fleshy and soft. Seeds 20–50, reniform, flattened and curved, 1.5–1.9 mm long and 1–1.7 mm wide; surface light yellow or brownish-yellow. Odour slight; taste sweet.

鉴别 Identification

(1)本品粉末黄橙色或红棕色。外果皮表皮细胞表面观呈类多角形或长多角形,垂周壁平直或细波状弯曲,外平周壁表面有平行的角质条纹。中果皮薄壁细胞呈类多角形,壁薄,胞腔内含橙红色或红棕色球形颗粒。种皮石细胞表面观不规则多角形,壁厚,波状弯曲,层纹清晰。
(1) The powder is yellow-orange or reddish-brown. In surface view, epidermal cells of the exocarp are polygonal or elongated polygonal; the anticlinal walls are straight or slightly wavy, and the outer periclinal walls show parallel cuticular striations. Parenchyma cells of the mesocarp are polygonal with thin walls, containing orange-red or reddish-brown spherical granules in the lumen. In surface view, sclereids (stone cells) of the testa are irregularly polygonal with thick walls, wavy contours, and distinct stratification.

(2)取本品 0.5 g,加水 35 ml,加热煮沸 15 分钟,放冷,滤过,滤液用乙酸乙酯 15 ml 振摇提取,分取乙酸乙酯液,浓缩至 1 ml,作为供试品溶液。另取枸杞子对照药材 0.5 g,同法制成对照药材溶液。照薄层色谱法(通则 0502)试验,吸取上述两种溶液各 5 μl,分别点于同一硅胶 G 薄层板上,以乙酸乙酯-三氯甲烷-甲酸(3∶2∶1)为展开剂,展开,取出,晾干,置紫外光灯(365 nm)下检视。供试品色谱中,在与对照药材色谱相应的位置上,显相同颜色的荧光斑点。
(2) Take 0.5 g of the sample, add 35 ml of water, heat to boiling for 15 minutes, cool and filter. Shake the filtrate with 15 ml of ethyl acetate, separate the ethyl acetate layer and concentrate to 1 ml to obtain the test solution. Prepare the reference drug solution from 0.5 g of Lycium Fruit reference material in the same manner. Perform thin-layer chromatography <0502>. Apply 5 μl of each solution to the same silica gel G TLC plate. Develop with ethyl acetate–chloroform–formic acid (3:2:1). Remove the plate, air-dry, and examine under UV light at 365 nm. In the chromatogram of the test solution, a fluorescent spot of the same colour appears at the corresponding position to that of the reference drug solution.

检查 Examination

水分 Water

不得过 13.0%(通则 0832 第二法,温度为 80 ℃)。
Not more than 13.0% <0832, Method 2>, at 80 ℃.

总灰分 Total ash

不得过 5.0%(通则 2302)。
Not more than 5.0% <2302>.

重金属及有害元素 Heavy metals and harmful elements

照铅、镉、砷、汞、铜测定法(通则 2321 原子吸收分光光度法或电感耦合等离子体质谱法)测定,铅不得过 5 mg/kg;镉不得过 0.3 mg/kg;砷不得过 2 mg/kg;汞不得过 0.2 mg/kg;铜不得过 20 mg/kg。
Determine by the method for lead, cadmium, arsenic, mercury, and copper <2321> (atomic absorption spectrophotometry or inductively coupled plasma mass spectrometry). Limits: lead, not more than 5 mg/kg; cadmium, not more than 0.3 mg/kg; arsenic, not more than 2 mg/kg; mercury, not more than 0.2 mg/kg; copper, not more than 20 mg/kg.

浸出物 Extractives

照水溶性浸出物测定法(通则 2201)项下的热浸法测定,不得少于 55.0%。
Determine by the method for water-soluble extractives <2201>, hot extraction method. Not less than 55.0%.

含量测定 Assay

枸杞多糖 Lycium Polysaccharides

对照品溶液的制备 Preparation of reference solution

取无水葡萄糖对照品 25 mg,精密称定,置 250 ml 量瓶中,加水适量溶解,稀释至刻度,摇匀,即得(每 1 ml 中含无水葡萄糖 0.1 mg)。
Accurately weigh 25 mg of anhydrous glucose reference substance, place in a 250 ml volumetric flask, dissolve in a suitable amount of water, dilute to volume, and mix well (each 1 ml contains 0.1 mg of anhydrous glucose).

标准曲线的制备 Preparation of standard curve

精密量取对照品溶液 0.2 ml、0.4 ml、0.6 ml、0.8 ml、1.0 ml,分别置具塞试管中,分别加水补至 2.0 ml,各精密加入 5% 苯酚溶液 1 ml,摇匀,迅速精密加入硫酸 5 ml,摇匀,放置 10 分钟,置 40 ℃ 水浴中保温 15 分钟,取出,迅速冷却至室温,以相应的试剂为空白,照紫外-可见分光光度法(通则 0401),在 490 nm 的波长处测定吸光度,以吸光度为纵坐标,浓度为横坐标,绘制标准曲线。
Accurately measure 0.2 ml, 0.4 ml, 0.6 ml, 0.8 ml, and 1.0 ml of the reference solution into separate stoppered test tubes, and add water to make each to 2.0 ml. Add 1 ml of 5% phenol solution accurately to each tube, mix well, then rapidly add 5 ml of sulfuric acid accurately and mix. Let stand for 10 minutes, keep in a 40 ℃ water bath for 15 minutes, remove and cool rapidly to room temperature. Using the corresponding reagent as blank, determine the absorbance at 490 nm by UV–Vis spectrophotometry <0401>. Plot the standard curve with absorbance as ordinate and concentration as abscissa.

测定法 Assay method

取本品粗粉约 0.5 g,精密称定,加乙醚 100 ml,加热回流 1 小时,静置,放冷,小心弃去乙醚液,残渣置水浴上挥尽乙醚。加入 80% 乙醇 100 ml,加热回流 1 小时,趁热滤过,滤渣与滤器用热 80% 乙醇 30 ml 分次洗涤,滤渣连同滤纸置烧瓶中,加水 150 ml,加热回流 2 小时。趁热滤过,用少量热水洗涤滤器,合并滤液与洗液,放冷,移至 250 ml 量瓶中,用水稀释至刻度,摇匀,精密量取 1 ml,置具塞试管中,加水 1.0 ml,照标准曲线的制备项下的方法,自“各精密加入 5% 苯酚溶液 1 ml”起,依法测定吸光度,从标准曲线上读出供试品溶液中含葡萄糖的重量(mg),计算,即得。
Accurately weigh about 0.5 g of the coarse powder. Add 100 ml of ether and reflux for 1 hour. Let stand, cool, carefully discard the ether, and evaporate residual ether from the residue on a water bath. Add 100 ml of 80% ethanol and reflux for 1 hour; filter while hot. Wash the residue and filter with 30 ml of hot 80% ethanol in portions. Transfer the residue together with the filter paper to a flask, add 150 ml of water, and reflux for 2 hours. Filter while hot, wash the filter with a small amount of hot water, combine filtrate and washings, cool, transfer to a 250 ml volumetric flask, dilute to volume with water, and mix well. Accurately measure 1 ml into a stoppered test tube and add 1.0 ml of water. Proceed as described under preparation of the standard curve starting from “add 1 ml of 5% phenol solution”, determine the absorbance, read the amount of glucose (mg) in the test solution from the standard curve, and calculate.

本品按干燥品计算,含枸杞多糖以葡萄糖(C6H12O6)计,不得少于 1.8%。
Calculated on the dried basis, the content of Lycium Polysaccharides, expressed as glucose (C6H12O6), should be not less than 1.8%.

甜菜碱 Betaine

供试品溶液的制备 Preparation of test solution

取本品剪碎,取约 2 g,精密称定,加 80% 甲醇 50 ml,加热回流 1 小时,放冷,滤过,用 80% 甲醇 30 ml 分次洗涤残渣和滤器,合并洗液与滤液,浓缩至 10 ml,用盐酸调节 pH 值至 1,加入活性炭 1 g,加热煮沸,放冷,滤过,用水 15 ml 分次洗涤,合并洗液与滤液,加入新配制的 2.5% 硫氰酸铬铵溶液 20 ml,搅匀,10 ℃ 以下放置 3 小时。用 G4 垂熔漏斗滤过,沉淀用少量冰水洗涤,抽干,残渣加丙酮溶解,转移至 5 ml 量瓶中,加丙酮至刻度,摇匀,作为供试品溶液。
Cut the sample into pieces, accurately weigh about 2 g, add 50 ml of 80% methanol, reflux for 1 hour, cool and filter. Wash the residue and filter with 30 ml of 80% methanol in portions, combine filtrate and washings, and concentrate to 10 ml. Adjust to pH 1 with hydrochloric acid, add 1 g of activated carbon, heat to boiling, cool and filter. Wash with 15 ml of water in portions, combine filtrate and washings, add 20 ml of freshly prepared 2.5% ammonium chromic thiocyanate solution, mix well, and stand below 10 ℃ for 3 hours. Filter through a G4 sintered-glass funnel, wash the precipitate with a small amount of ice water, and dry by suction. Dissolve the residue in acetone, transfer to a 5 ml volumetric flask, dilute to volume with acetone, mix well, and use as the test solution.

对照品溶液的制备 Preparation of reference solution

另取甜菜碱对照品适量,精密称定,加盐酸甲醇溶液(0.5→100)制成每 1 ml 含 4 mg 的溶液,作为对照品溶液。
Accurately weigh an appropriate amount of betaine reference substance, and dissolve in methanolic hydrochloric acid solution (0.5→100) to make a solution containing 4 mg per ml as the reference solution.

薄层色谱-扫描测定 TLC with densitometric scanning

照薄层色谱法(通则 0502)试验,精密吸取供试品溶液 5 μl、对照品溶液 3 μl 与 6 μl,分别交叉点于同一硅胶 G 薄层板上,以丙酮-无水乙醇-盐酸(10∶6∶1)为展开剂,预饱和 30 分钟,展开,取出,挥干溶剂,立即喷以新配制的改良碘化铋钾试液,放置 1~3 小时至斑点清晰,照薄层色谱法(通则 0502)进行扫描,波长:λS=515 nm,λR=590 nm,测量供试品吸光度积分值与对照品吸光度积分值,计算,即得。
Perform thin-layer chromatography <0502>. Accurately apply 5 μl of the test solution and 3 μl and 6 μl of the reference solution as crossed spots on the same silica gel G TLC plate. Develop with acetone–absolute ethanol–hydrochloric acid (10:6:1) after pre-saturation for 30 minutes. Remove the plate, evaporate the solvent, immediately spray with freshly prepared modified potassium bismuth iodide reagent, and allow to stand for 1–3 hours until the spots are clear. Scan as described in <0502> with wavelengths λS = 515 nm and λR = 590 nm. Measure the integrated absorbance of the test spot and the reference spot(s) and calculate.

本品按干燥品计算,含甜菜碱(C5H11NO2)不得少于 0.30%。
Calculated on the dried basis, the content of betaine (C5H11NO2) should be not less than 0.30%.

性味与归经 Properties and meridian tropism

甘,平。归肝、肾经。
Sweet, neutral. Enters the Liver and Kidney meridians.

功能与主治 Actions and indications

滋补肝肾,益精明目。用于虚劳精亏,腰膝酸痛,眩晕耳鸣,阳萎遗精,内热消渴,血虚萎黄,目昏不明。
Nourishes the liver and kidney, benefits essence, and improves vision. Used for consumptive deficiency with insufficiency of essence, soreness and weakness of the waist and knees, dizziness and tinnitus, impotence and seminal emission, internal heat with thirst, blood deficiency with sallow complexion, and blurred vision.

用法与用量 Administration and dosage

6~12 g。
6–12 g.

贮藏 Storage

置阴凉干燥处,防闷热,防潮,防蛀。
Store in a cool and dry place; protect from heat, moisture, and insect infestation.


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MLMD百科MLMD Encyclopedia

植物学信息 Botanical Information

本天然药材为茄科植物宁夏枸杞Lycium barbarum L.的干燥成熟果实。
This product is the dried mature fruit of the Solanaceae plant Lycium barbarum L., also known as Ningxia wolfberry.

加工炮制 Processing and Preparation

夏、秋二季果实呈红色时采收,热风烘干,除去果梗,或晾至皮皱后,晒干,除去果梗。
The fruit is harvested in summer and autumn when it turns red, then dried with hot air and the fruit stem is removed. Alternatively, it is air-dried until the skin wrinkles, then sun-dried and the fruit stem is removed.


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成分Ingredients
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相关靶点Related Targets
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中
En
由NMM所含成分推断,具有成分-靶点关系的文献证据。
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中
En
由NMM所含成分推断,具有成分-疾病关系的文献证据。
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