北刘寄奴

中文文本参考：《中国药典（2020年版）》
English text reference: Chinese Pharmacopoeia (2020 Edition)

概述 Overview

本品为玄参科植物阴行草Siphonostegia chinensis Benth.的干燥全草。秋季采收，除去杂质，晒干。
Siphonostegia Herb is the dried whole plant of Siphonostegia chinensis Benth. (Fam. Labiatae). The drug is collected in autumn, removed from impurities, and dried.

性状 Description

本品长30～80 cm，全体被短毛。根短而弯曲，稍有分枝。茎圆柱形，有棱，有的上部有分枝，表面棕褐色或黑棕色；质脆，易折断，断面黄白色，中空或有白色髓。叶对生，多脱落破碎，完整者羽状深裂，黑绿色。总状花序顶生，花有短梗，花萼长筒状，黄棕色至黑棕色，有明显10条纵棱，先端5裂，花冠棕黄色，多脱落。蒴果狭卵状椭圆形，较萼稍短，棕黑色。种子细小。气微，味淡。
Stems 30-80 cm long, covered with short hairs. Root short and curved, slightly branched. Stem cylindrical, with ridges, some upper parts branched, surface brownish-brown or blackish-brown; texture brittle, easily broken, fracture yellowish-white, hollow or with white pith. Leaves opposite, mostly shed and broken, intact ones pinnately deeply lobed, blackish-green. Inflorescence terminal, flowers with short pedicels, calyx tubular, yellowish-brown to blackish-brown, with 10 obvious longitudinal ridges, apex 5-lobed, corolla brownish-yellow, mostly shed. Capsule narrowly ovoid-elliptical, slightly shorter than calyx, brownish-black. Seeds small. Odour, faint; taste, mild.

鉴别 Identification

（1）本品茎横切面：表皮可见非腺毛，非腺毛2～4细胞。皮层由2～4列细胞组成。中柱鞘纤维成环状。韧皮部较窄。形成层不明显。木质部10余列，由导管和木纤维组成，射线细胞单列。髓薄壁细胞排列紧密，有的细胞具细密的纹孔。
(1) Transverse section of stem: Non-glandular hairs visible in the epidermis, 2-4 cells. Cortex composed of 2-4 rows of cells. Middle sheath fibers arranged in a ring. Phloem relatively narrow. Cambium indistinct. Xylem more than 10 rows, composed of vessels and fibers, ray cells single. Pith thin-walled cells arranged closely, some cells with fine pits.

（2）取本品粉末2 g，加甲醇20 ml，超声处理30分钟，滤过，滤液浓缩至1 ml，作为供试品溶液。另取木犀草素对照品，加甲醇制成每1 ml含1 mg的溶液，作为对照品溶液。照薄层色谱法（通则0502）试验，吸取上述两种溶液各5 μl，分别点于同一硅胶G薄层板上，以甲苯-甲酸乙酯-甲酸（5:4:1）为展开剂，展开，取出，晾干，喷以1%三氯化铝试液，在105℃加热数分钟，置紫外光灯（365 nm）下检视。供试品色谱中，在与对照品色谱相应的位置上，显相同颜色的荧光斑点。
(2) Take 2 g of the powder, add 20 ml of methanol, sonicate for 30 minutes, filter, concentrate the filtrate to 1 ml as the test solution. Take resveratrol CRS, add methanol to make a solution containing 1 mg per ml as the reference solution. Carry out the method for thin layer chromatography<0502>, using silica gel G as the coating substance and a mixture of toluene, ethyl acetate, and formic acid (5:4:1) as the mobile phase. Apply separately to the plate 5 μl of each of the above two solutions. After developing and removal of the plate, dry in air. Spray with a 1% solution of ferric chloride in ethanol, heat at 105°C for several minutes, examine under ultraviolet light at 365 nm. The fluorescent spot in the chromatogram obtained with the test solution corresponds in position and colour to the spot in the chromatogram obtained with the reference solution.

检查 Examination

水分 Water

不得过12.0%（通则0832第二法）。
Not more than 12.0 per cent <0832,method 2>.

总灰分 Total ash

不得过8.0%（通则2302）。
Not more than 8.0 per cent <2302>.

浸出物 Extractives

照醇溶性浸出物测定法（通则2201）项下的热浸法测定，用70%乙醇作溶剂，不得少于10.0%。
Carry out the method for determination of ethanol-soluble Extractives <2201,the hot maceration method>, using 70% ethanol as the solvent,not less than 10.0 per cent.

含量测定 Content determination

照高效液相色谱法（通则0512）测定。
Carry out the method for determination of content by high performance liquid chromatography <0512>.

色谱条件与系统适用性试验 Chromatographic conditions and system suitability test

以十八烷基硅烷键合硅胶为填充剂；以甲醇为流动相A，以0.05%磷酸溶液为流动相B；按下表中的规定进行梯度洗脱；毛蕊花糖苷检测波长为310 nm，木犀草素检测波长为350 nm。理论板数按毛蕊花糖苷峰计算应不低于3000。
Use octadecylsilane bonded silica gel as the filler; use methanol as mobile phase A and 0.05% phosphoric acid solution as mobile phase B; perform gradient elution according to the specified conditions in the table; the detection wavelength for verbascoside is 310 nm, and the detection wavelength for luteolin is 350 nm. The theoretical plate number calculated based on the peak of verbascoside should not be less than 3000.

对照品溶液的制备 Preparation of reference solution

取木犀草素对照品、毛蕊花糖苷对照品适量，精密称定，加甲醇制成每1 ml含木犀草素70 μg、毛蕊花糖苷0.25 mg的混合溶液，即得。
Take an appropriate amount of luteolin reference substance and verbascoside reference substance, accurately weigh, add methanol to make a mixed solution containing 70 μg of luteolin and 0.25 mg of verbascoside per 1 ml.

供试品溶液的制备 Preparation of test solution

取本品粉末（过二号筛）约2 g，精密称定，置具塞锥形瓶中，精密加入85%甲醇25 ml，称定重量，加热回流1.5小时，放冷，再称定重量，用85%甲醇补足减失的重量，摇匀，滤过，取续滤液，即得。
Take about 2 g of the powder of this product (passed through a No. 2 sieve), accurately weigh, place it in a stoppered conical flask, accurately add 25 ml of 85% methanol, weigh, heat under reflux for 1.5 hours, cool, weigh again, make up for the weight loss with 85% methanol, shake well, filter, and take the filtrate.

测定法 Determination method

分别精密吸取对照品溶液与供试品溶液各5 μl，注入液相色谱仪，测定，即得。
Accurately draw 5 μl of the reference solution and the test solution, respectively, inject them into the liquid chromatograph, and determine.

本品按干燥品计算，含木犀草素（C15H10O6）不得少于0.050%；含毛蕊花糖苷（C29H36O15）不得少于0.060%。
Calculated on the dried product, the content of luteolin (C15H10O6) should not be less than 0.050%, and the content of verbascoside (C29H36O15) should not be less than 0.060%.

饮片 Prepared slices

北刘寄奴 Siphonostegia Herb

炮制 Processing

除去杂质，洗净，切段，干燥。
Eliminate Foreign matter,wash, cut into sections, and dry in the sun.

性状 Description

本品呈不规则的段。茎呈圆柱形，有棱，表面棕褐色或黑棕色，被短毛。切面黄白色，中空或有白色髓。花萼长筒状，黄棕色至黑棕色，有明显10条纵棱，先端5裂。蒴果狭卵状椭圆形，较萼稍短，棕黑色，种子细小。
The product is in irregular segments. The stem is cylindrical, angular, with a tan or black-brown surface, covered with short hairs. The cut surface is yellowish white, hollow or with white pith. Calyx long tubular, yellowish brown to blackish brown, with 10 distinct longitudinal ribs, apex 5-lobed. Capsule narrowly ovate-elliptic, slightly shorter than calyx, brownish black, seeds minute.

鉴别 Identification

（除茎横切面外）同药材。
(Except stem cross section) Same as the crude drug.

检查 Examination

同药材。
Same as the crude drug.

浸出物 Extractives

同药材。
Same as the crude drug.

含量测定 Assay

同药材。
Same as the crude drug.

性 Property

寒。
Cold.

味 Flavor

苦。
Bitter.

归经 Meridian tropism

归脾、胃、肝、胆经。
Spleen, stomach, liver, and gallbladder meridians.

功能 Actions

活血祛瘀，通经止痛，凉血，止血，清热利湿。
To activate blood circulation and remove blood stasis, regulate menstruation and relieve pain, cool blood, stop bleeding, clear heat and promote diuresis.

主治 Indications

用于跌打损伤，外伤出血，瘀血经闭，月经不调，产后瘀痛，癥瘕积聚，血痢，血淋，湿热黄疸，水肿腹胀，白带过多。
Used for injuries from falls, external bleeding, blood stasis and amenorrhea, irregular menstruation, postpartum stasis pain, accumulation of masses, bloody dysentery, hematuria, damp-heat jaundice, edema and abdominal distension, excessive leukorrhea.

用量 Dosage

6～9 g。
6-9 g.

用法 Administration

无。
None.

贮藏 Storage

置干燥处。
Store in a dry place.

北刘寄奴

文本参考：《中国药典（2015年版）》
Text reference: Chinese Pharmacopoeia (2015 Edition)

概述 Overview

本品为玄参科植物阴行草Siphonostegia chinensis Benth.的干燥全草。秋季采收，除去杂质，晒干。
Chinensis Siphonostegia Herb is the dried herb of Siphonostegia chinensis Benth. (Fam. Scroph-ulariaceae). The drug is collected in autumn, removed from foreign matter, and dried in the sun.

性状 Description

本品长30～80cm，全体被短毛。根短而弯曲，稍有分枝。茎圆柱形，有棱，有的上部有分枝，表面棕褐色或黑棕色；质脆，易折断，断面黄白色，中空或有白色髓。叶对生，多脱落破碎，完整者羽状深裂，黑绿色。总状花序顶生，花有短梗，花萼长筒状，黄棕色至黑棕色，有明显10条纵棱，先端5裂，花冠棕黄色，多脱落。蒴果狭卵状椭圆形，较萼稍短，棕黑色。种子细小。气微，味淡。
30-80 cm long, shortly pubscent. Roots short and curved, slightly branched. Stems cylindrical, with ridges, sometimes the upper part branched, externally brown or blackish-brown; texture fragile, easily broken, fracture yellowish-white, hollowed or with white pith. Leaves opposite, mostly fallen off and broken, when whole, pinnatipartite, blackish-green. Racemes acrogenous, flowers with short pedicel, calyx long-tubular, yellowish-brown to blackish-brown, with 10 distinct longitudinal ridges, 5-lobed at the apex, corolla brownish-yellow, mostly fallen off. Capsules narrow-ovoid-elliptical, slightly shorter than calyx, brownish-black. Seeds minute. Odour, slight; taste, weak.

鉴别 Identification

（1）本品茎横切面：表皮可见非腺毛，非腺毛2～4细胞。皮层由2～4列细胞组成。中柱鞘纤维成环状。韧皮部较窄。形成层不明显。木质部10余列，由导管和木纤维组成，射线细胞单列。髓薄壁细胞排列紧密，有的细胞具细密的纹孔。
（2）取本品粉末2g，加甲醇20ml，超声处理30分钟，滤过，滤液浓缩至1ml，作为供试品溶液。另取木犀草素对照品，加甲醇制成每1ml含1mg的溶液，作为对照品溶液。照薄层色谱法（通则0502）试验，吸取上述两种溶液各5μl，分别点于同一硅胶G薄层板上，以甲苯-甲酸乙酯-甲酸（5：4：1）为展开剂，展开，取出，晾干，喷以1%三氯化铝试液，在105℃加热数分钟，置紫外光灯（365nm）下检视。供试品色谱中，在与对照品色谱相应的位置上，显相同颜色的荧光斑点。

检查 Examination

水分 Water

不得过12.0%（通则0832第二法）。
Not more than 12.0 per cent <0832, method 2>.

总灰分 Total ash

不得过8.0%（通则2302）。
Not more than 8.0 per cent <2302>.

浸出物 Extractives

照醇溶性浸出物测定法（通则2201）项下的热浸法测定，用70%乙醇作溶剂，不得少于10.0%。
Carry out the method for determination of ethanol-soluble extractives <2201, the hot extraction method>, using 70% ethanol as the solvent, not less than 10.0 per cent.

含量测定 Assay

照高效液相色谱法（通则0512）测定。
Carry out the method for high performance liquid chromatography <0512>.

色谱条件与系统适用性试验 Chromatographic system and system suitability

以十八烷基硅烷键合硅胶为填充剂；以甲醇为流动相A，以0.05%磷酸溶液为流动相B；按下表中的规定进行梯度洗脱；毛蕊花糖苷检测波长为310nm，木犀草素检测波长为350mn。理论板数按毛蕊花糖苷峰计算应不低于3000。

时间（分钟） 流动相A（%） 流动相B（%）

0～15
33
6715～30
33→60
67→40
30～40
60
40

对照品溶液的制备
取木犀草素对照品、毛蕊花糖苷对照品适量，精密称定，加甲醇制成每1ml含木犀草素70μg、毛蕊花糖苷0.25mg的混合溶液，即得。

饮片 Prepared slices

北刘寄奴 Chinensis Siphonostegia

炮制 Processing

除去杂质，洗净，切段，干燥。
Eliminate foreign matter, wash clean, cut into sections, and dry.

本品呈不规则的段。茎呈圆柱形，有棱，表面棕褐色或黑棕色，被短毛。切面黄白色，中空或有白色髓。花萼长筒状，黄棕色至黑棕色，有明显10条纵棱，先端5裂。蒴果狭卵状椭圆形，较萼稍短，棕黑色，种子细小。
In irregular sections. Stems cylindrical, with ridges, externally brown or blackish-brown, shortly pubescent. Fracture yellowish-white, hollowed or with white medulla. Calyx long-tubular, yellowish-brown to blackish-brown, with 10 distinct longitudinal ridges, 5-lobed at the apex. Capsules narrow ovoid-elliptical, slightly shorter than calyx, brownish-black, seeds minute.

性 Property

苦，寒。
Cold, bitter.

味 Flavor

苦，寒。
Bitter, cold.

归经 Meridian tropism

归脾、胃、肝、胆经。
Spleen, stomach, liver and gallbladder meridians.

功能 Actions

活血祛瘀，通经止痛，凉血，止血，清热利湿。
To activate, and eliminate stasis, unblock the meridians, relieve pain, cool the blood, stanch bleeding, clear heat and drain dampness.

主治 Indications

用于跌打损伤，外伤出血，瘀血经闭，月经不调，产后瘀痛，癥瘕积聚，血痢，血淋，湿热黄疸，水肿腹胀，白带过多。
Traumatic injuries, traumatic bleeding, blood-stasis amenorrhea, menstrual irregularities, postpartum stasis and pain, abdominal masses, blood dysentery, blood strangury, dampness-heat jaundice, edema, abdominal distension, and profuse white vaginal discharge.

用量 Dosage

6～9g。
6-9 g.

用法 Administration

无特殊要求。
No special requirements.

贮藏 Storage

置干燥处。
Preserve in a dry place.