续断

中文文本参考：《中国药典（2020年版）》
English text reference: Chinese Pharmacopoeia (2020 Edition)

概述 Overview

本品为川续断科植物川续断Dipsacus asper Wall. Ex Henry的干燥根。秋季采挖，除去根头和须根，用微火烘至半干，堆置“发汗”至内部变绿色时，再烘干。
Dipsacus Root is the dried root of Dipsacus asper Wall. Ex Henry (Fam. Dipsacaceae). The drug is collected in autumn, removed from root head and rootlet, dried with gentle heat until semi-dry, and then piled up for "sweating" until the internal part turns green, and finally dried.

性状 Description

本品呈圆柱形，略扁，有的微弯曲，长5～15cm，直径0.5～2cm。表面灰褐色或黄褐色，有稍扭曲或明显扭曲的纵皱及沟纹，可见横列的皮孔样斑痕和少数须根痕。质软，久置后变硬，易折断，断面不平坦，皮部墨绿色或棕色，外缘褐色或淡褐色，木部黄褐色，导管束呈放射状排列。气微香，味苦、微甜而后涩。
Roots cylindrical, slightly flattened, some slightly curved, 5-15 cm long, 0.5-2 cm in diameter; surface grayish-brown or yellowish-brown, with slightly twisted or obviously twisted longitudinal wrinkles and grooves, visible transverse rows of pock-like scars and a few rootlet scars. Texture soft, hardens after long storage, easily broken, uneven fracture surface, epidermis dark green or brown, outer edge brown or light brown, wood yellowish-brown, vascular bundles arranged radially. Odour, slightly aromatic; taste, bitter, slightly sweet, and then astringent.

鉴别 Identification

（1）本品横切面：木栓细胞数列。栓内层较窄。韧皮部筛管群稀疏散在。形成层环明显或不甚明显。木质部射线宽广，导管近形成层处分布较密，向内渐稀少，常单个散在或2～4个相聚。髓部小，细根多无髓。薄壁细胞含草酸钙簇晶。
(1)Transverse section of the product: cork cells in several rows. Inner layer of cork is narrow. Groups of sieve tubes in the phloem sparsely scattered. Formation layer ring obvious or less obvious. Xylem rays are broad, and ducts are densely distributed near the formation layer, becoming sparse inwards, often scattered singly or in two to four clusters. The pith is small, and fine roots are mostly without pith. Thin-walled cells contain calcium oxalate clusters.

粉末黄棕色。草酸钙簇晶甚多，直径15～50μm，散在或存在于皱缩的薄壁细胞中，有时数个排列成紧密的条状。纺锤形薄壁细胞壁稍厚，有斜向交错的细纹理。具缘纹孔导管和网纹导管直径约至72（90）μm。木栓细胞淡棕色，表面观类长方形、类方形、多角形或长多角形，壁薄。
Powder yellowish-brown. Numerous clusters of calcium oxalate crystals, measuring 15-50 μm in diameter, scattered or present in shriveled parenchyma cells, sometimes arranged in several rows in a compact strip. Spindle-shaped parenchyma cells with slightly thickened walls and oblique, interlacing fine striations. Pits with a beaded border and reticulate vessels, with a diameter of about 72 (90) μm. Cork cells pale brown, surface view rectangular, square, polygonal or elongated polygonal, thin-walled.

（2）取本品粉末3g，加浓氨试液4ml，拌匀，放置1小时，加三氯甲烷30ml，超声处理30分钟，滤过，滤液用盐酸溶液（4→100）30ml分次振摇提取，提取液用浓氨试液调节pH值至10，再用三氯甲烷20ml分次振摇提取，合并三氯甲烷液，浓缩至0.5ml，作为供试品溶液。另取续断对照药材3g，同法制成对照药材溶液。照薄层色谱法（通则0502）试验，吸取上述两种溶液各5μl，分别点于同一硅胶G薄层板上，以乙醚-丙酮（1：1）为展开剂，展开，取出，晾干，喷以改良碘化铋钾试液。供试品色谱中，在与对照药材色谱相应的位置上，显相同颜色的斑点。
（2）To 3g of the powder add 4ml of concentrated ammonia TS, mix well, let stand for 1 hour, add 30ml of chloroform, treat with ultrasound for 30 minutes, filter, extract the filtrate with 30ml of hydrochloric acid solution (4→100) by shaking, adjust the pH value of the extract with concentrated ammonia TS to 10, then extract with 20ml of chloroform by shaking, combine the chloroform solution, concentrate to 0.5ml, and use it as the test solution. Take an appropriate amount of Dipsacus Root as the reference material, and prepare a solution of the reference material in the same way. Carry out the method for thin layer chromatography<0502>, using silica gel G as the coating substance and a mixture of ethyl acetate and acetone (1:1) as the mobile phase. Apply separately to the plate 5 μl of each of the above two solutions. After developing and removal of the plate, dry in air. Spray with a 10% solution of sulfuric acid in ethanol, heat at 105°C to the spots clear. The spots in the chromatogram obtained with the test solution correspond in position and colour to the spots in the chromatogram obtained with the reference material.

（3）取本品粉末0.2g，加甲醇15ml，超声处理30分钟，滤过，滤液蒸干，残渣加甲醇2ml使溶解，作为供试品溶液。另取川续断皂苷VI对照品，加甲醇制成每1ml含1mg的溶液，作为对照品溶液。照薄层色谱法（通则0502）试验，吸取上述两种溶液各5μl，分别点于同一硅胶G薄层板上，以正丁醇-醋酸-水（4︰1︰5）的上层溶液为展开剂，展开，取出，晾干，喷以10%硫酸乙醇溶液，加热至斑点显色清晰。供试品色谱中，在与对照品色谱相应的位置上，显相同颜色的斑点。
（3）To 0.2g of the powder add 15ml of methanol, treat with ultrasound for 30 minutes, filter, evaporate the filtrate to dryness, dissolve the residue in 2ml of methanol, and use it as the test solution. Take an appropriate amount of Dipsacus saponin VI CRS, and prepare a solution of the reference material containing 1mg per ml in the same way. Carry out the method for thin layer chromatography<0502>, using silica gel G as the coating substance and a mixture of n-butanol, acetic acid, and water (4:1:5) as the upper layer solution. Apply separately to the plate 5 μl of each of the above two solutions. After developing and removal of the plate, dry in air. Spray with a 10% solution of sulfuric acid in ethanol, heat at 105°C to the spots clear. The spots in the chromatogram obtained with the test solution correspond in position and colour to the spots in the chromatogram obtained with the reference material.

检查 Examination

水分 Water

不得过10.0%（通则0832第二法）。
Not more than 10.0 per cent <0832,method 2>.

总灰分 Total ash

不得过12.0%（通则2302）。
Not more than 12.0 per cent <2302>.

酸不溶性灰分 Acid-insoluble ash

不得过3.0%（通则2302）。
Not more than 3.0 per cent <2302>.

浸出物 Extractives

照水溶性浸出物测定法（通则2201）项下的热浸法测定，不得少于45.0%。
Carry out the method for determination of water-soluble Extractives <2201,the hot maceration method>, not less than 45.0 per cent.

含量测定 Content determination

照高效液相色谱法（通则0512）测定。
Carry out the method for high performance liquid chromatography<0512>.

色谱条件与系统适用性试验 Chromatographic conditions and system suitability test

以十八烷基硅烷键合硅胶为填充剂；以乙腈-水（30︰70）为流动相；检测波长为212nm。理论板数按川续断皂苷VI峰计算应不低于3000。
Chromatographic conditions and system suitability test: Use octadecylsilane-bonded silica gel as the filling material; use acetonitrile-water (30:70) as the mobile phase; detect at a wavelength of 212 nm. The theoretical plate number calculated based on the peak of Dipsacus saponin VI should not be less than 3000.

对照品溶液的制备 Preparation of reference solution

取川续断皂苷VI对照品适量，精密称定，加甲醇制成每1ml含1.5mg的溶液。精密量取1ml，置10ml量瓶中，加流动相稀释至刻度，摇匀，即得。
Preparation of the reference material solution: Take an appropriate amount of Dipsacus saponin VI CRS, accurately weigh, add methanol to prepare a solution containing 1.5mg per ml. Take an accurate amount of 1ml, place it in a 10ml volumetric flask, dilute with the mobile phase to the mark, shake well, and use.

供试品溶液的制备 Preparation of test solution

取本品细粉约0.5g，精密称定，置具塞锥形瓶中，精密加入甲醇25ml，密塞，称定重量，超声处理（功率100W，频率40kHz）30分钟，放冷，再称定重量，用甲醇补足减失的重量，摇匀，滤过，精密量取续滤液5ml，置50ml量瓶中，加流动相稀释至刻度，摇匀，即得。
Preparation of the test solution: Take about 0.5g of the powder, accurately weigh, place it in a stoppered conical flask, accurately add 25ml of methanol, seal tightly, weigh, treat with ultrasound (power 100W, frequency 40kHz) for 30 minutes, cool, weigh again, make up for the weight loss with methanol, shake well, filter, take an accurate amount of 5ml of the filtrate, place it in a 50ml volumetric flask, dilute with the mobile phase to the mark, shake well, and use.

测定法 Assay method

分别精密吸取对照品溶液与供试品溶液各20μl，注入液相色谱仪，测定，即得。
Method of determination: Accurately take 20μl of the reference material solution and the test solution, inject into the liquid chromatograph, and determine.

本品按干燥品计算，含川续断皂苷VI（C47H76O18）不得少于2.0%。
Calculated on the dried product, the content of Dipsacus saponin VI (C47H76O18) should not be less than 2.0%.

饮片 Prepared slices

续断片 Dipsacus Slices

炮制 Processing

洗净，润透，切厚片，干燥。
Clean, soak until moist, cut into thick slices, and dry.

性状 Description

本品呈类圆形或椭圆形的厚片。外表皮灰褐色至黄褐色，有纵皱。切面皮部墨绿色或棕褐色，木部灰黄色或黄褐色，可见放射状排列的导管束纹，形成层部位多有深色环。气微，味苦、微甜而涩。
This product is in thick slices of similar round or oval shape. The epidermis is greyish brown to yellowish brown with longitudinal wrinkles. The cut surface of the skin is dark green or brown, and the wood is greyish yellow or yellowish brown, with radially arranged conduit bundles and dark rings in the formation layer. Gas slightly, taste bitter, slightly sweet and astringent.

含量测定 Assay

同药材，含川续断皂苷VI（C47H76O18）不得少于1.5%。
Same as the crude drug，containing not less than 1.5% Dipsacus saponin VI (C47H76O18).

鉴别 Identification

（除横切面外）同药材。
(except cross sections)Same as the crude drug.

检查 Examination

同药材。
Same as the crude drug.

浸出物 Extractives

同药材。
Same as the crude drug.

酒续断 Dipsacus (processed with wine)

炮制 Processing

取续断片，照酒炙法（通则0213）炒至微带黑色。
Take the slices of Sequence, according to the wine-roasted method ，<0213> fried until slightly black.

性状 Description

本品形如续断片，表面浅黑色或灰褐色，略有酒香气。
This product is shaped like a piece of Sequence, the surface is light black or grey-brown, with a slight aroma of wine.

含量测定 Assay

同药材，含川续断皂苷VI（C47H76O18）不得少于1.5%。
Same as the crude drug，containing not less than 1.5% Dipsacus saponin VI (C47H76O18).

鉴别 Identification

（除横切面外）同药材。
(except cross sections)Same as the crude drug.

检查 Examination

同药材。
Same as the crude drug.

浸出物 Extractives

同药材。
Same as the crude drug.

盐续断 Dipsacus (processed with salt-water)

炮制 Processing

取续断片，照盐炙法（通则0213）炒干。
Take the slices of Sequitoria, according to the salt-roasted method < 0213>, fried and dried.

性状 Description

本品形如续断片，表面黑褐色，味微咸。
TThis product is shaped like sequestra tablets, with a dark brown surface and a slightly salty taste.

含量测定 Assay

同药材，含川续断皂苷VI（C47H76O18）不得少于1.5%。
Same as the crude drug，containing not less than 1.5% Dipsacus saponin VI (C47H76O18).

鉴别 Identification

（除横切面外）同药材。
(except cross sections)Same as the crude drug.

检查 Examination

同药材。
Same as the crude drug.

浸出物 Extractives

同药材。
Same as the crude drug.

性 Property

微温。
Slightly warm.

味 Flavor

苦、辛。
Bitter, pungent.

归经 Meridian tropism

归肝、肾经。
Liver and kidney meridians.

功能 Actions

补肝肾，强筋骨，续折伤，止崩漏。
To nourish the liver and kidney, strengthen tendons and bones, heal fractures, and stop excessive uterine bleeding.

主治 Indications

用于肝肾不足，腰膝酸软，风湿痹痛，跌扑损伤，筋伤骨折，崩漏，胎漏。酒续断多用于风湿痹痛，跌扑损伤，筋伤骨折。盐续断多用于腰膝酸软。
Used for liver and kidney deficiency, soreness and weakness of the waist and knees, rheumatic arthralgia, falls and injuries, tendon injuries and fractures, excessive uterine bleeding, and leakage during pregnancy. Wine-processed Dipsacus Root is mainly used for rheumatic arthralgia, falls and injuries, and tendon injuries and fractures. Salt-processed Dipsacus Root is mainly used for soreness and weakness of the waist and knees.

用量 Dosage

9～15g。
9-15 g.

用法 Administration

无。
None.

贮藏 Storage

置干燥处，防蛀。
Store in a dry place, protect from moth.

概述 | Overview

本品为川续断科植物川续断Dipsacus asper Wall. ex Henry的干燥根。秋季采挖，除去根头和须根，用微火烘至半干，堆置“发汗”至内部变绿色时，再烘干。
Himalayan Teasel Root is the dried root of Dipsacus asper Wall.ex Henry (Fam. Dipsacaceae). The drug is collected in autumn, removed from the root stock and rootlet, baked to half-dryness, piled up until a green colour is developed inside, and then baked to dryness.

性状 | Description

本品呈圆柱形，略扁，有的微弯曲，长5～15cm，直径0.5～2cm。表面灰褐色或黄褐色，有稍扭曲或明显扭曲的纵皱及沟纹，可见横列的皮孔样斑痕和少数须根痕。质软，久置后变硬，易折断，断面不平坦，皮部墨绿色或棕色，外缘褐色或淡褐色，木部黄褐色，导管束呈放射状排列。气微香，味苦、微甜而后涩。
Cylindrical, somewhat flattened, some slightly curved, 5-15 cm long, 0.5-2 cm in diameter. Externally greyish-brown or yellowish-brown, with slightly twisted or obviously twisted longitudinal wrinkles and furrows, showing transversal lenticel-like cicatrices and sparse rootlet scars. Texture soft and hardened after long storage, easily broken, fracture uneven, bark dark green or brown, the outer part brown or pale brown; wood yellowish-brown, vessel bundles arranged radially. Odour, slightly aromatic; taste, bitter, slightly sweet, then astringent.

鉴别 | Identification

（1）本品横切面：木栓细胞数列。栓内层较窄。韧皮部筛管群稀疏散在。形成层环明显或不甚明显。木质部射线宽广，导管近形成层处分布较密，向内渐稀少，常单个散在或2～4个相聚。髓部小，细根多无髓。薄壁细胞含草酸钙簇晶。
粉末黄棕色。草酸钙簇晶甚多，直径15～50μm，散在或存在于皱缩的薄壁细胞中，有时数个排列成紧密的条状。纺锤形薄壁细胞壁稍厚，有斜向交错的细纹理。具缘纹孔导管和网纹导管直径约至72（90）μm，木栓细胞淡棕色，表面观类长方形、类方形、多角形或长多角形，壁薄。
（2）取本品粉末3g，加浓氨试液4ml，拌匀，放置1小时，加三氯甲烷30ml，超声处理30分钟，滤过，滤液用盐酸溶液（4→100）30ml分次振摇提取，提取液用浓氨试液调节pH值至10，再用三氯甲烷20ml分次振摇提取，合并三氯甲烷液，浓缩至0.5ml，作为供试品溶液。另取续断对照药材3g，同法制成对照药材溶液。照薄层色谱法（通则0502）试验，吸取上述两种溶液各5μl，分别点于同一硅胶G薄层板上，以乙醚-丙酮（1：1）为展开剂，展开，取出，晾干，喷以改良碘化铋钾试液。供试品色谱中，在与对照药材色谱相应的位置上，显相同颜色的斑点。
（3）取本品粉末0.2g，加甲醇15ml，超声处理30分钟，滤过，滤液蒸干，残渣加甲醇2ml使溶解，作为供试品溶液。另取川续断皂苷Ⅵ对照品，加甲醇制成每1ml含1mg的溶液，作为对照品溶液。照薄层色谱法（通则0502）试验，吸取上述两种溶液各5μl，分别点于同一硅胶G薄层板上，以正丁醇-醋酸-水（4：1：5）的上层溶液为展开剂，展开，取出，晾干，喷以10%硫酸乙醇溶液，加热至斑点显色清晰。供试品色谱中，在与对照品色谱相应的位置上，显相同颜色的斑点。
To 3 g of the powder add 50 ml of petroleum ether (60-90°C), heat under reflux for 1 hour, filter, evaporate the filtrate to dryness,dissolve the residue in 30 ml of n-hexane. Extract with three 10-ml quantities of 20%acetonitrile solution,evaporate the combined extracts to dryness. Dissolve the residue in 0.5 ml of ethanol as the test solution. Dissolve artemisinin CRS in ethanol to produce a solution containing 1 mg per ml as the Reference solution . Carry out the method for thin layer chromatography<0502>, using silica gel G as the coating substance and a mixture of petroleum ether(60-90°C)and ether(4:5)as the mobile phase. Apply separately to the plate 5 μl of each of the above two solutions. After developing and removal of the plate,dry in air. Spray with a 2% solution of vanillin in 10%sulfuric acid in ethanol, heat at 105°C to the spots clear. Examine under ultraviolet light at 365 nm. The fluorescent spot in the chromatogram obtained with the test solution corresponds in position and colour to the spot in the chromatogram obtained with the Reference solution.

检查 | Examination

水分 | Water

不得过10.0%（通则0832第二法）。
Not more than 10.0 per cent <0832, method 2>.

总灰分 | Total ash

不得过12.0%（通则2302）。
Not more than 12.0 per cent <2302>.

酸不溶性灰分 | Acid-insoluble ash

不得过3.0%（通则2302）。
Not more than 3.0 per cent <2302>.

浸出物 | Extractives

照水溶性浸出物测定法（通则2201）项下的热浸法测定，不得少于45.0%。
Carry out the method for determination of water-soluble extractives <2201, the hot extraction method>, not less than 45.0 per cent.

含量测定 | Assay

照高效液相色谱法（通则0512）测定。
Carry out the method for high performance liquid chromatography <0512>.

饮片 | Prepared slices

续断 | Himalayan Teasel

炮制 | Processing

续断片洗净，润透，切厚片，干燥。
Radir Dipsaci (sliced) Wash clean, soften thoroughly, cut into thin slices, and dry.

本品呈类圆形或椭圆形的厚片。外表皮灰褐色至黄褐色，有纵皱。切面皮部墨绿色或棕褐色，木部灰黄色或黄褐色，可见放射状排列的导管束纹，形成层部位多有深色环。气微，味苦、微甜而涩。
In subrounded or elliptical thick slices. Externally, greyish-brown to yellowish-brown, with longitudinal wrinkles. In cut surface, bark dark green or brown, wood greyish-yellow or yellowish-brown, showing radial lines consisting of vessel groups, the part of cambium exhibiting a dark-coloured ring. Odour slight; taste bitter, slightly sweet and astringent.

含量测定 | Assay

同药材，含川续断皂苷Ⅵ（C47H76O18）不得少于1.5%。
Same as the crude drug, it contains not less than 1.5% of asperosaponin VI (CHO).

鉴别 | Identification

（除横切面外）【检查】【浸出物】同药材。
Identification: (except for transverse section)【Examination】【Extractives】Same as the crude drug.

酒续断 | Himalayan Teasel processed with wine

取续断片，照酒炙法（通则0213）炒至微带黑色。
Dipsaci Radix (processed with wine) Stir-bake the slicesof Radix Dipsaci as described under the method for stir-baking with wine 〈0213〉 to be blackish.

本品形如续断片，表面浅黑色或灰褐色，略有酒香气。
The shape similar to the slices, externally pale black or greyish-brown, slightly with wine aromatic odour.

含量测定 | Assay

同药材，含川续断皂苷Ⅵ（C47H76O18）不得少于1.5%。
Same as the crude drug, it contains not less than 1.5% of asperosaponin VI (CHO).

鉴别 | Identification

（除横切面外）【检查】【浸出物】同药材。
Identification: (except for transverse section)【Examination】【Extractives】Same as the crude drug.

盐续断 | Himalayan Teasel processed with salt-water

取续断片，照盐炙法（通则0213）炒干。
Dipsaci Radix (processed with salt-water) Stir-bake the slices of Radix Dipsaci as described under the method for stir-baking with salt-water 〈0213〉 to dryness.

本品形如续断片，表面黑褐色，味微咸。
The shape similar to the slices, externally blackish-brown, taste slightly salty.

含量测定 | Assay

同药材，含川续断皂苷Ⅵ（C47H76O18）不得少于1.5%。
Same as the crude drug, it contains not less than 1.5% of asperosaponin VI (CHO).

鉴别 | Identification

（除横切面外）【检查】【浸出物】同药材。
Identification: (except for transverse section)【Examination】【Extractives】Same as the crude drug.

性味与归经 | Property and Flavor

苦、辛，微温。归肝、肾经。
Mild warm; bitter and pungent. Liver and kidney meridians.

功能与主治 | Actions and Indications

补肝肾，强筋骨，续折伤，止崩漏。用于肝肾不足，腰膝酸软，风湿痹痛，跌扑损伤，筋伤骨折，崩漏，胎漏。酒续断多用于风湿痹痛，跌扑损伤，筋伤骨折。盐续断多用于腰膝酸软。
To tonify liver-kidney, strengthen sinew and bone, reconnect fracture, stop bleeding. Used for deficiency of liver-kidney, soreness and weakness in the lower back and knees, painful bi disorder caused by wind-dampness, traumatic injuries, sinew injury and fracture, menstrual flooding and spotting, vaginal bleeding during pregnancy; Dipsaci Radix (processed with wine) is usually used for painful impediment caused by wind-dampness, injuries from falls, sinew injury and fracture. Dipsaci Radix (processed with salt) is usually used for soreness and weakness in the lower back and knees.

用法与用量 | Administration and dosage

9～15g。
9-15 g.

贮藏 | Storage

置干燥处，防蛀。
Preserve in a dry place, and protect from moth.