紫花前胡

English text reference: Chinese Pharmacopoeia (2020 Edition)

Overview

Peucedani Decursivi Radix is the dried root of Peucedanum decursivum (Miq.) Maxim. (Fam. Umbelliferae). The drug is collected in autumn and winter when the aerial part withers, removed from rootlets, and dried in the sun.

Description

Roots irregularly cylindrical, conical or fusiform, main root slender, with a few lateral roots, 3-15cm long, 0.8-1.7cm in diameter; surface brown to dark brown, with occasional remains of stem base and membranous leaf sheath, shallowly longitudinally wrinkled, with grayish-white transverse lenticels-like projections and dotted rootlet scars. Texture hard, fracture white, cortex narrow, with a few yellow oil dots. Odour, aromatic; taste, slightly bitter and pungent.

Identification

（1）Cross-section of the root of this product: the cork layer is several columns to more than 10 rows of flat cells, with an epicortical layer outside. The inner layer of the plug is extremely narrow, and there are tubing scattered in it. Phloem broad; Most of the tubing are round, slightly arranged in a multi-wheel ring shape, and there are 5~10 secretory cells; Phloem rays are often curved and form fissures of varying sizes near the cortex. The cambium is ring-shaped. The xylem is small, and the ducts are arranged radially in a radial pattern; Wood rays are wider; Wood fibers are rare. Parenchyma cells contain starch granules.

（2）To 0.5g of the powder add 25ml of methanol, treat with ultrasound for 20 minutes, filter, and use the filtrate as the test solution. Prepare a solution containing 50μg per ml of peucedanin CRS in methanol as the Reference solution. Carry out the method for thin layer chromatography<0502>, using silica gel G as the coating substance and a mixture of ethyl acetate-methanol-water (8:1:1) as the mobile phase. Apply separately to the plate 5μl of each of the above two solutions. After developing and removal of the plate,dry in air. Examine under ultraviolet light at 365 nm. The fluorescent spot in the chromatogram obtained with the test solution corresponds in position and colour to the spot in the chromatogram obtained with the Reference solution.

Examination

Water

Not more than 12.0 per cent <0832,method 2>.

Total ash

Not more than 8.0 per cent <2302>.

Acid-insoluble ash

Not more than 4.0 per cent <2302>.

Extractives

Carry out the method for determination of ethanol-soluble Extractives <2201,the hot maceration method>, using diluted ethanol as the solvent,not less than 30.0 per cent.

Content determination

Carry out the method for high performance liquid chromatography<0512>.

Chromatographic conditions and system suitability test

Use octadecylsilane bonded silica gel as the filler; use methanol-water (40:60) as the mobile phase; detect at the wavelength of 334nm. The theoretical plate number calculated from the peak of peucedanin should not be less than 1500.

Preparation of reference solution

Take an appropriate amount of peucedanin CRS, accurately weigh, add methanol to make a solution containing 50μg per ml, and mix well.

Preparation of test solution

Take about 0.5g of the powder (passed through a No. 3 sieve) of the test drug, accurately weigh, place it in a stoppered conical flask, accurately add 25ml of methanol, weigh again, soak for 1 hour, treat with ultrasound (power 100W, frequency 40kHz) for 20 minutes, cool, weigh again, make up the loss in weight with methanol, shake well, filter, accurately measure 1 ml of the filtrate, place it in a 10ml volumetric flask, add methanol to the mark, shake well, and obtain the test solution.

Determination method

Accurately draw 5μl of each of the reference solution and the test solution, inject into the liquid chromatograph, and determine.

Calculated on the dried basis, the content of peucedanin (C20H24O9) should not be less than 0.90%.

Prepared slices

Peucedani Decursivi Radix

Processing

Eliminate Foreign matter, wash clean, soften briefly, cut into thin slices, and dry in the sun.

Property

Slightly cold.

Flavor

Bitter and pungent.

Meridian tropism

Lung meridian.

Actions

To descend qi and transform phlegm, dispel wind and clear heat.

Indications

Used for phlegm-heat cough with fullness in the chest, thick and yellow sputum, and cough with phlegm due to wind-heat.

Dosage

3-9g.

Administration

Or made into pills or powder.

Storage

Preserve in a cool and dry place, protect from mold and moth.

紫花前胡

Text reference: Chinese Pharmacopoeia (2015 Edition)

Overview

Decurrent Hogfennel Root is the dried root and rhizome of Peucedanum decursivum (Miq.) Maxim. (Fam. Umbelliferae). The drug is collected in autumn to winter when the aerial part withered, removed from rootlet, and dried in the sun.

Description

Irregular cylindrical, conical or fusiform, taproots relatively thin, with a few of branches, 3-15 cm long, 0.8-1.7 cm in diameter. Externally brown to blackish-brown, with shallow and straight fine longitudinal wrinkles, occasionally with remains of stems and membranous leaf sheaths at the root stock, greyish-white transverse lenticel-like protrudings and dotted rootlet scars visible. Texture hard, fracture whitish, bark part relatively narrow, scattered with a few of yellow oily spots. Odour, aromatic; taste slightly bitter and pungent.

Identification

（2）取本品粉末0.5g，加甲醇25ml，超声处理20分钟，滤过，取滤液作为供试品溶液。另取紫花前胡苷对照品，加甲醇制成每1ml含50μg的溶液，作为对照品溶液。照薄层色谱法（通则0502）试验，吸取上述两种溶液各5μl，分别点于同一硅胶G薄层板上，以乙酸乙酯-甲醇-水（8：1：1）为展开剂，展开，取出，晾干，置紫外光灯（365nm）下检视。供试品色谱中，在与对照品色谱相应的位置上，显相同颜色的荧光斑点。

Examination

Water

Not more than 12.0 per cent <0832, method 2>.

Total ash

Not more than 8.0 per cent <2302>.

Acid-insoluble ash

Not more than 4.0 per cent <2302>.

Extractives

Carry out the method for determination of ethanol-soluble extractives <2201, the hot extraction method>, using dilute ethanol as the solvent, not less than 30.0 per cent.

Assay

Carry out the method for high-performance liquid chromatography <0512>.

Chromatographic system and system suitability

Use octadecylsilane bonded silica gel as the stationary phase and a mixture of methanol and water (40:60) as the mobile phase. As a detector, a spectrophotometer set at 334 nm. The number of theoretical plates of the column is not less than 1500, calculated with reference to the peak of nodakenin.

Preparation of the reference solution

Dissolve a quantity of nodakenin CRS in methanol to produce a solution containing 50 μg per ml as the reference solution.

Preparation of the test solution

Weigh accurately 0.5 g of the powder (through No. 3 sieve) to a stoppered conical flask, accurately add 25 ml of methanol and weigh. Macerate for 1 hour and ultrasonicate (power 100 W, frequency 40 kHz) for 20 minutes, cool and weigh again, replenish the loss of weight with methanol, mix well, and filter. Measure accurately 1 ml of the subsequent filtrate to a 10 ml volumetric flask, dilute with methanol to volume, and mix well.

Procedure

Inject accurately 5 μl of each of the reference solution and the test solution, respectively, into the column, and calculate the content.

It contains not less than 0.90 per cent of nodakenin (C20H24O9), calculated with reference to the dried drug.

Prepared slices

Decurrent Hogfennel

Processing

Eliminate foreign matters, wash clean, soften thoroughly, cut into thin slices, and dry in the sun.

Property

Slightly cold.

Flavor

Bitter and pungent.

Meridian tropism

Lung meridian.

Actions

To direct qi downward, resolve phlegm, disperse wind, and clear heat.

Indications

Phlegm-heat wheezing and fullness, yellow thick phlegm, wind-heat cough, and profuse sputum.

Dosage

3-9 g, or used in pills and powder.

Administration

Add when the decoction is nearly done.

Storage

Preserve in a cool and dry place, and protect from mould and moth.