洪连

English text reference: *Chinese Pharmacopoeia (2020 Edition) *
中文文本参考：《中国药典（2020年版）》

Overview 概述

Lagotidis Herb is the dried aerial part of Lagotis brevituba Maxim. (Fam. Scrophulariaceae). The drug is collected in summer and autumn at flowering, removed from impurities, washed, and dried in the shade.
本品系藏族习用药材。为玄参科植物短筒兔耳草Lagotis brevituba Maxim.的干燥全草。夏、秋二季花开时采收，除去杂质，洗净，阴干。

Description 性状

The drug is 5-15 cm long. Rhizome cylindrical, slightly curved, internodes close together, resembling silkworm; surface grayish-brown or light purplish-brown; texture brittle, easily broken, fracture brownish or grayish-yellow, with 3-4 white dot-like vascular bundles arranged in a ring. Roots slender, cylindrical, twisted, surface light yellowish-brown or grayish-brown, with longitudinal wrinkles. Radical leaves with long petioles; leaf blades crumpled and broken, when whole, circular or ovate, blunt at apex, margin with rounded teeth, base broadly cuneate. Spike-like inflorescence terminal. Fruit elongate-ovoid, blackish-brown. Odour, slight; taste, slightly bitter.
本品长5～15 cm。根茎呈圆柱形，略弯曲，节间紧密，形似蚕；表面灰褐色或浅紫褐色；质脆，易折断，断面棕褐色或灰黄色，有3～4个白色的点状维管束，排列成环。根细长，圆柱形，扭曲，表面浅黄褐色或灰褐色，有纵皱纹。基生叶，具长柄；叶片多卷曲破碎，完整者展平后呈圆形或卵圆形，先端钝圆，边缘具圆齿，基部宽楔形。穗状花序顶生。果长圆形，黑褐色。气微，味微苦。

Identification 鉴别

（1）The powder is reddish-brown. Numerous starch grains, single grains are round, with a diameter of 3-7 μm, occasionally helmet-shaped, with a dot-like hilum; compound grains consist of 2-3 (6) component grains. Thin-walled cells are round or round-like, containing light brown round-like nuclear substances. Epidermal cells of the lower surface of the leaf have slightly curved periclinal walls, stomata are anomocytic and anisocytic. Vessels are mostly reticulate and spiral.
（1）本品粉末红棕色。淀粉粒众多，单粒类圆形，直径3～7 μm，偶见盔帽形，脐点点状；复粒由2～3（6）分粒组成。薄壁细胞圆形或类圆形，内含浅棕色类圆形核状物。叶下表皮细胞垂周壁稍弯曲，气孔不定式和不等式。导管多为网纹导管和螺纹导管。

（2）To 0.5 g of the powder add 10 ml of methanol, treat with ultrasound for 15 minutes, filter, and use the filtrate as the test solution. Prepare separately a solution of the reference substances, arctiin and verbascoside, in methanol to contain 1 mg per ml as the Reference solution. Carry out the method for thin layer chromatography <0502>, using polyamide film as the coating substance and a mixture of methanol-acetic acid-water (2: 1: 7) as the mobile phase. Apply separately to the plate 2 μl of each of the above three solutions. After developing and removal of the plate, dry in air. Examine under ultraviolet light at 365 nm. The fluorescent spot in the chromatogram obtained with the test solution corresponds in position and colour to the spot in the chromatogram obtained with the Reference solution.
（2）取本品粉末0.5 g，加甲醇10 ml，超声处理15分钟，滤过，滤液作为供试品溶液。另取松果菊苷对照品、毛蕊花糖苷对照品，加甲醇分别制成每1 ml含1 mg的溶液，作为对照品溶液。照薄层色谱法（通则0502）试验，吸取上述三种溶液各2 μl，分别点于同一聚酰胺薄层板上，以甲醇-醋酸-水（2∶1∶7）为展开剂，展开，取出，晾干，置紫外光灯（365 nm）下检视。供试品色谱中，在与对照品色谱相应的位置上，显相同颜色的荧光斑点。

Examination 检查

Water 水分

Not more than 8.0 per cent <0832, method 2>.
不得过8.0%（通则0832第二法）。

Total ash 总灰分

Not more than 15.0 per cent <2302>.
不得过15.0%（通则2302）。

Acid-insoluble ash 酸不溶性灰分

Not more than 10.0 per cent <2302>.
不得过10.0%（通则2302）。

Extractives 浸出物

Carry out the method for determination of ethanol-soluble Extractives <2201, the cold maceration method>, using ethanol as the solvent, not less than 8.0 per cent.
照醇溶性浸岀物测定法（通则2201）项下的冷浸法测定，用乙醇作溶剂，不得少于8.0%。

Assay 含量测定

Carry out the method for high performance liquid chromatography <0512>.
照高效液相色谱法（通则0512）测定。

Chromatographic conditions and system suitability test 色谱条件与系统适用性试验

Use octadecylsilane bonded silica gel as the filler; use acetonitrile-methanol-1% acetic acid solution (10: 15: 75) as the mobile phase; detection wavelength is 334 nm. The theoretical plate number calculated from the peak of arctiin should not be less than 4000.
以十八烷基硅烷键合硅胶为填充剂；以乙腈-甲醇-1%醋酸溶液（10∶15∶75）为流动相;检测波长为334 nm。理论板数按松果菊苷峰计算应不低于4000。

Preparation of reference solution 对照品溶液的制备

Take an appropriate amount of arctiin reference substance, accurately weigh, place it in a brown volumetric flask, add the mobile phase to make a solution containing 0.25 mg per ml, and mix well.
取松果菊苷对照品适量，精密称定，置棕色量瓶中，加流动相制成每1 ml含0.25 mg的溶液，即得。

Preparation of test solution 供试品溶液的制备

Take about 0.5 g of the powder (passed through a No. 4 sieve) of the test substance, accurately weigh, place it in a 50 ml brown volumetric flask, accurately add 25 ml of the mobile phase, weigh, soak for 30 minutes, treat with ultrasound (power 230 W, frequency 35 kHz) for 15 minutes, cool, weigh again, make up for the lost weight with the mobile phase, shake well, centrifuge, let stand, take the supernatant and place it in a brown bottle, and obtain the test solution.
取本品粉末（过四号筛）约0.5 g，精密称定，置50 ml棕色量瓶中，精密加入流动相25 ml，称定重量，浸泡30分钟，超声处理（功率230 W，频率35 kHz）15分钟，放冷，再称定重量，用流动相补足减失的重量，摇匀，离心，静置，取上清液置棕色瓶中，即得。

Assay method 测定法

Accurately draw 10-20 μl of the reference solution and the test solution, respectively, inject into the liquid chromatograph, and determine.
分别精密吸取对照品溶液与供试品溶液各10～20 μl，注入液相色谱仪，测定，即得。

Calculated on the dried basis, the content of arctiin (C33H46O20) should not be less than 0.80%.
本品按干燥品计算，含松果菊苷（C33H46O20）不得少于0.80%。

Property 性

Cold.
寒。

Flavor 味

Bitter and sweet.
苦、甘。

Meridian tropism 归经

Lung, heart, and liver meridians.
归肺、心、肝经。

功能 ｜ Actions

To clear heat, detoxify, promote diuresis, soothe the liver, promote blood circulation, and regulate menstruation.
清热，解毒，利湿，平肝，行血，调经。

Indications 主治

Used for fever and thirst, lung heat cough, headache and dizziness, damp-heat jaundice, irregular menstruation, and poisoning by drugs and food.
用于发热烦渴，肺热咳嗽，头痛眩晕，湿热黄疸，月经不调，药食中毒。

Dosage 用量

1-6 g.
1～6 g。

用法 ｜ Administration

None.
无。

Storage 贮藏

Preserve in a well-ventilated and dry place.
置通风干燥处。

洪连

Text reference: Chinese Pharmacopoeia (2015 Edition)
文本参考：《中国药典（2015年版）》

Overview 概述

Shorttube Lagotis Herb is the dried herb of Lagotis brevituba Maxim. (Fam. Scrophulariaceae), commonly used by the Tibetan nationality. The drug is collected at flowering season in summer and autumn, removed from foreign matter, washed clean, and dried in the shade.
本品系藏族习用药材。为玄参科植物短筒兔耳草Lagotis brevituba Maxim.的干燥全草。夏、秋二季花开时采收，除去杂质，洗净，阴干。

Description 性状

5-15 cm long. Rhizomes cylindrical, slightly curved, internodes very short like a silkworm; externally greyish-brown or pale purplish-brown; texture fragile, easily broken, fracture brown or greyish-yellow, 3-4 white dotted vascular bundles arranged in a ring. Roots slender, cylindrical twisted, externally pale yellowish-brown or greyish-brown, with longitudinal wrinkles. Leaves basal, long petioled; lamina mostly rolled and broken, when whole, rounded or ovoid, apex obtuse, margin rounded-serrate, base broadly cuneate. Spike terminal. Fruits oblong, blackish-brown. Odour, slight; taste, slightly bitter.
本品长5～15cm。根茎呈圆柱形，略弯曲，节间紧密，形似蚕；表面灰褐色或浅紫褐色；质脆，易折断，断面棕褐色或灰黄色，有3～4个白色的点状维管束，排列成环。根细长，圆柱形，扭曲，表面浅黄褐色或灰褐色，有纵皱纹。基生叶，具长柄；叶片多卷曲破碎，完整者展平后呈圆形或卵圆形，先端钝圆，边缘具圆齿，基部宽楔形。穗状花序顶生。果长圆形 ，黑褐色。气微，味微苦。

Identification 鉴别

（2）取本品粉末0.5g，加甲醇10ml，超声处理15分钟，滤过，滤液作为供试品溶液。另取松果菊苷对照品、毛蕊花糖苷对照品，加甲醇分别制成每1ml含1mg的溶液，作为对照品溶液。照薄层色谱法（通则0502）试验，吸取上述三种溶液各2μl，分别点于同一聚酰胺薄层板上，以甲醇-醋酸-水（2：1：7）为展开剂，展开，取出，晾干，置紫外光灯（365nm）下检视。供试品色谱中在与对照品色谱相应的位置上，显相同颜色的荧光斑点。
（1）本品粉末红棕色。淀粉粒众多，单粒类圆形，直径 3～7μm，偶见盔帽形，脐点点状；复粒由 2～3（6）分粒组成。薄壁细胞圆形或类圆形，内含浅棕色类圆形核状物。叶下表皮细胞垂周壁稍弯曲，气孔不定式和不等式。导管多为网纹导管和螺纹导管。

Examination 检查

Water 水分

Not more than 8.0 per cent <0832, method 2>.
不得过8.0%（通则0832第二法）。

Total ash 总灰分

Not more than 15.0 per cent <2302>.
不得过15.0%（通则2302）。

Acid-insoluble ash 酸不溶性灰分

Not more than 10.0 per cent <2302>.
不得过10.0%（通则2302）。

Extractives 浸出物

Carry out the method for determination of ethanol-soluble extractives <2201, the cold maceration method>, using ethanol as the solvent, not less than 8.0 per cent.
照醇溶性浸出物测定法（通则2201）项下的冷浸法测定，用乙醇作溶剂，不得少于8.0%。

Assay 含量测定

Carry out the method for high-performance liquid chromatography <0512>.
照高效液相色谱法（通则0512）测定。

Storage 贮藏

Preserve in a ventilated and dry place.
置通风干燥处。