香加皮

English text reference: Chinese Pharmacopoeia (2020 Edition)
中文文本参考：《中国药典（2020年版）》

Overview 概述

Periploca Bark is the dried root bark of Periploca sepium Bge. (Fam. Asclepiadaceae). The drug is collected in spring and autumn, the root bark is peeled off, and dried in the shade.
本品为萝藦科植物杠柳Periploca sepium Bge.的干燥根皮。春、秋二季采挖，剥取根皮，晒干。

Description 性状

The bark is cylindrical or trough-shaped, occasionally irregularly blocky, 3-10 cm long, 1-2 cm in diameter, and 0.2-0.4 cm thick. The outer surface is grayish-brown or yellowish-brown, with loose cork often in scales that are easily peeled off. The inner surface is pale yellow or pale yellowish-brown, relatively smooth, with fine longitudinal striations. The bark is light, brittle, easily broken, with an uneven fracture surface, and yellowish-white. It has a characteristic aroma and a bitter taste.
本品呈卷筒状或槽状，少数呈不规则的块片状，长3～10cm，直径1～2cm，厚0.2～0.4cm。外表面灰棕色或黄棕色，栓皮松软常呈鳞片状，易剥落。内表面淡黄色或淡黄棕色，较平滑，有细纵纹。体轻，质脆，易折断，断面不整齐，黄白色。有特异香气，味苦。

Identification 鉴别

(1) The powder of this product is light brown. Calcium oxalate square crystals 9-20 μm in diameter. stone cells oblong or polygonal-like, 24-70 μm in diameter. milk tubes containing colourless oil droplet-like particles. Cork cells are brownish yellow, polygonal. Starch granules are numerous, single granules are oblong or oblong, 3～l1μm in diameter; compound granules are composed of 2～6 grains.
（1）本品粉末淡棕色。草酸钙方晶直径9～20μm。石细胞长方形或类多角形，直径24～70μm。乳管含无色油滴状颗粒。木栓细胞棕黄色，多角形。淀粉粒甚多，单粒类圆形或长圆形，直径3～l1μm；复粒由2～6分粒组成。

(2) Take 10g of this product powder, placed in 250ml flask, add 150ml of water, heating distillation, distillate with a specific aroma, collect 10ml of distillate, divided into two test tubes, a tube with 1% ferric chloride solution 1 drop, that is, a reddish-brown colour; the other tube with a small amount of hydrazine sulphate saturated solution 5ml and sodium acetate crystals, a little heated, cooled to produce a yellowish-green precipitate, and placed in ultraviolet light (365nm) under the observation, showed strong yellow fluorescence.
（2）取本品粉末10g，置250ml烧瓶中，加水150ml，加热蒸馏，馏出液具特异香气，收集馏出液10ml，分置二支试管中，一管中加1%三氯化铁溶液1滴，即显红棕色；另一管中加硫酸肼饱和溶液5ml与醋酸钠结晶少量，稍加热，放冷，生成淡黄绿色沉淀，置紫外光灯（365nm）下观察，显强烈的黄色荧光。

(3) Take 1g of this product powder, add 10ml of ethanol, heat and reflux for 1 hour, filtration, filtrate in a 25ml measuring flask, add ethanol to the scale, shaking well, a precise amount of 1ml, placed in a 20ml measuring flask, add ethanol to the scale, shaking well, according to the UV-visible spectrophotometric method (General 0401) to determine the wavelength of 278nm has the maximum absorption.
（3）取本品粉末1g，加乙醇10ml，加热回流1小时，滤过，滤液置25ml量瓶中，加乙醇至刻度，摇匀，精密量取1ml，置20ml量瓶中，加乙醇至刻度，摇匀，照紫外-可见分光光度法（通则0401）测定，在278nm的波长处有最大吸收。

(4) Take 2g of this product powder, add 30ml of methanol, heating reflux for 1 hour, filtration, filtrate evaporation, residue add 2ml of methanol to dissolve, as a test solution. Take 4-methoxysalicylaldehyde control product, add methanol to make a solution containing lmg per 1ml, as a control solution. According to the thin layer chromatography (General rule 0502) test, absorb the above two solutions of 2μl, respectively, point on the same silica gel G thin layer plate, with petroleum ether (60 ~ 90 ℃) - ethyl acetate - glacial acetic acid (20:3:0.5) as the unfolding agent, unfold, remove, dry, spray with dinitrophenylhydrazine test solution. In the chromatogram of the test article, spots of the same colour are shown in the corresponding positions with the chromatogram of the control article.
（4）取本品粉末2g，加甲醇30ml，加热回流1小时，滤过，滤液蒸干，残渣加甲醇2ml使溶解，作为供试品溶液。另取4-甲氧基水杨醛对照品，加甲醇制成每1ml含lmg的溶液，作为对照品溶液。照薄层色谱法（通则0502）试验，吸取上述两种溶液各2μl，分别点于同一硅胶G薄层板上，以石油醚（60～90℃）-乙酸乙酯-冰醋酸（20∶3∶0.5）为展开剂，展开，取出，晾干，喷以二硝基苯肼试液。供试品色谱中，在与对照品色谱相应的位置上，显相同颜色的斑点。

Examination 检查

Water 水分

Not more than 13.0 per cent <0832,method 2>.
不得过13.0%（通则0832第二法）。

Total ash 总灰分

Not more than 10.0 per cent <2302>.
不得过10.0%（通则2302）。

Acid-insoluble ash 酸不溶性灰分

Not more than 4.0 per cent <2302>.
不得过4.0%（通则2302）。

Extractives 浸出物

Carry out the method for determination of ethanol-soluble Extractives <2201,the hot maceration method>, using diluted ethanol as the solvent, not less than 20.0 per cent.
照醇溶性浸出物测定法（通则2201）项下的热浸法测定，用稀乙醇作溶剂，不得少于20.0%。

Assay 含量测定

Carry out the method for high performance liquid chromatography <0512>.
照高效液相色谱法（通则0512）测定。

Chromatographic conditions and system suitability test 色谱条件与系统适用性试验

Use octadecylsilane bonded silica gel as the stationary phase; use methanol-water-acetic acid (70:30:2) as the mobile phase; detect at a wavelength of 278 nm. The theoretical plate number calculated based on the peak of 4-methoxysalicylaldehyde should not be less than 1000.
以十八烷基硅烷键合硅胶为填充剂；以甲醇-水-醋酸（70∶30∶2）为流动相；检测波长为278nm。理论板数按4-甲氧基水杨醛峰计算应不低于1000。

Preparation of internal standard solution 内标溶液的制备

Take an appropriate amount of butylparaben, accurately weigh, and dissolve in 60% methanol to obtain a solution containing 6 mg per ml.
取对羟基苯甲酸丁酯适量，精密称定，加60%甲醇制成每1ml含6mg的溶液，即得。

Assay method 测定法

Take an appropriate amount of 4-methoxysalicylaldehyde reference substance, accurately weigh, place it in a brown volumetric flask, and dissolve it in 60% methanol to obtain a solution containing 1 mg per ml. Accurately measure 4 ml of this solution and 2 ml of the internal standard solution, place them in a 25 ml volumetric flask, add 60% methanol to the mark, shake well, take 20 μl, inject it into the liquid chromatograph, and record the chromatogram. Take about 0.25-0.5 g of the coarse powder of the drug, dry it at 60°C for 4 hours, accurately weigh it, place it in a 50 ml flask, add 15 ml of 60% methanol, heat under reflux for 1.5 hours, filter, collect the filtrate in a 25 ml volumetric flask, wash the container with a small amount of 60% methanol, and filter the washings into the same flask. Accurately add 2 ml of the internal standard solution, add 60% methanol to the mark, shake well, filter, and take the subsequent filtrate as the test solution. Take 20 μl and inject it into the liquid chromatograph. Calculate the content based on the peak area using the internal standard method.
取4-甲氧基水杨醛对照品适量，精密称定，置棕色量瓶中，加60%甲醇制成每1ml含1mg的溶液。精密量取该溶液4ml、内标溶液2ml，置25ml量瓶中，加60%甲醇至刻度，摇匀，吸取20μl，注入液相色谱仪，记录色谱图；另取本品粗粉约0.25～0.5g，60℃干燥4小时，精密称定，置50ml烧瓶中，加60%甲醇15ml，加热回流1.5小时，滤过，滤液置25ml量瓶中，用少量60%甲醇洗涤容器，洗液滤入同一量瓶中，精密加入内标溶液2ml，加60%甲醇至刻度，摇匀，滤过，取续滤液作为供试品溶液。吸取20μl注入液相色谱仪，按内标法以峰面积计算，即得。

When dried at 60°C for 4 hours, the content of 4-methoxysalicylaldehyde (C8H8O3) should not be less than 0.20%.
本品于60℃干燥4小时，含4-甲氧基水杨醛（C8H8O3）不得少于0.20%。

Prepared slices 饮片

Periploca Bark 香加皮

Processing 炮制

Eliminate Foreign matter, wash, soften briefly, cut into thick slices, and dry in the sun.
除去杂质，洗净，润透，切厚片，干燥。

Description 性状

This product comes in thick irregular sheets. The outer surface is greyish brown or yellowish brown, and the embolus is often scaly. The inner surface is yellowish or yellowish brown with fine longitudinal lines. The cut surface is yellowish white. It has special aroma and bitter taste.
本品呈不规则的厚片。外表面灰棕色或黄棕色，栓皮常呈鳞片状。内表面淡黄色或淡黄棕色，有细纵纹。切面黄白色。有特异香气，味苦。

Identification 鉴别

Same as the crude drug.
同药材。

Examination 检查

Same as the crude drug.
同药材。

Assay 含量测定

Same as the crude drug.
同药材。

Property 性

Warm.
温。

Toxicity 毒性

Toxic.
有毒。

Flavor 味

Pungent and bitter.
辛、苦。

Meridian tropism 归经

Liver, kidney, and heart meridians.
归肝、肾、心经。

Actions 功能

To promote diuresis, reduce swelling, dispel wind and dampness, and strengthen tendons and bones.
利水消肿，祛风湿，强筋骨。

Indications 主治

Used for lower limb edema, palpitations and shortness of breath, wind-cold-damp arthralgia, and lumbago and knee weakness.
用于下肢浮肿，心悸气短，风寒湿痹，腰膝酸软。

Dosage 用量

3-6 g.
3～6g。

Administration 用法

None.
无。

Precautions 注意

Do not take excessive doses.
不宜过量服用。

Storage 贮藏

Preserve in a cool and dry place.
置阴凉干燥处。

香加皮

Text reference: Chinese Pharmacopoeia (2015 Edition)
文本参考：《中国药典（2015年版）》

Overview 概述

Chinese Silkvine Root-bark is the dried root bark of Periploca sepium Bge. (Fam. Asclepiadaceae ). The root is collected in spring and autumn, the root bark is stripped off, and dried in the sun.
本品为萝藦科植物杠柳Periploca sepium Bge.的干燥根皮。春、秋二季采挖，剥取根皮，晒干。

Description 性状

Quilled or channelled, a few pieced irregularly, 3-10 cm long, 1-2 cm in diameter, 2-4 mm thick. Outer surface greyish-brown or yellowish-brown, cork soft and loose, often scaly, easily exfoliated; inner surface pale yellow or pale yellowish-brown, relatively smooth, with fine longitudinal striations. Texture light and fragile, easily broken, fracture uneven, yellowish-white. Odour, characteristic and aromatic; taste, bitter.
本品呈卷筒状或槽状，少数呈不规则的块片状，长3～10cm，直径1～2cm，厚0.2～0.4cm。外表面灰棕色或黄棕色，栓皮松软常呈鱗片状，易剥落。内表面淡黄色或淡黄棕色，较平滑，有细纵纹。体轻，质脆，易折断，断面不整齐，黄白色。有特异香气，味苦。

Identification 鉴别

（2）取本品粉末10g，置250ml烧瓶中，加水150ml，加热蒸馏，馏出液具特异香气，收集馏出液10ml，分置二支试管中，一管中加1%三氯化铁溶液1滴，即显红棕色；另一管中加硫酸肼饱和溶液5ml与醋酸钠结晶少量，稍加热，放冷，生成淡黄绿色沉淀，置紫外光灯（365nm）下观察，显强烈的黄色荧光。
（1）本品粉末淡棕色。草酸钙方晶直径9～20μm。石细胞长方形或类多角形，直径24～70μm。乳管含无色油滴状颗粒。木栓细胞棕黄色，多角形。淀粉粒甚多，单粒类圆形或长圆形，直径3～11μm；复粒由2～6分粒组成。

Examination 检查

Water 水分

Not more than 13.0 per cent <0832, method 2>.
不得过13.0%（通则0832第二法）。

Total ash 总灰分

Not more than 10.0 per cent <2302>.
不得过10.0%（通则2302）。

Acid-insoluble ash 酸不溶性灰分

Not more than 4.0 per cent <2302>.
不得过4.0%（通则2302）。

Extractives 浸出物

Carry out the method for determination of ethanol-soluble Extractives <2201, the hot extraction method>, using dilute ethanol as the solvent, not less than 20.0 per cent.
照醇溶性浸出物测定法（通则2201）项下的热浸法测定，用稀乙醇作溶剂，不得少于20.0%。

Prepared slices 饮片

Chinese Silkvine Root-bark 香加皮

Processing 炮制

Eliminate Foreign matter, wash clean, soften thoroughly, cut into thick slices, and dry in the sun.
除去杂质，洗净，润透，切厚片，干燥。

Property 性

Warm.
温。

Flavor 味

Pungent and bitter.
辛、苦。

Meridian tropism 归经

Liver, kidney and heart meridians.
归肝、肾、心经。

Actions 功能

To promote urination, alleviate edema, dispel wind-dampness, and strengthen sinew and bone.
利水消肿，祛风湿，强筋骨。

Indications 主治

Edema in the lower limbs, palpitation, shortness of breath, wind cold dampness disorder, and soreness and weakness in the lower back and knees.
用于下肢浮肿，心悸气短，风寒湿痹，腰膝酸软。

Dosage 用量

3-6 g.
3～6g。

Precautions and Warnings 注意

Avoid overdosage.
不宜过量服用。

Storage 贮藏

Preserve in a cool and dry place.
置阴凉干燥处。