滇鸡血藤

中文文本参考：《中国药典（2020年版）》
English text reference: Chinese Pharmacopoeia (2020 Edition)

概述 Overview

本品为木兰科植物内南五味子Kadsura interior A. C. Smith的干燥藤茎。秋季采收，除去枝叶，切片，晒干。
Kadsura Stem is the dried stem of Kadsura interior A. C. Smith (Fam. Magnoliaceae). The drug is collected in autumn, removed from branches and leaves, sliced, and dried.

性状 Description

本品呈圆形、椭圆形或不规则的斜切片，直径1.8～6.5cm。表面灰棕色，栓皮剥落处呈暗红紫色，栓皮较厚，粗者具多数裂隙，呈龟裂状；细者具纵沟，常附有苔类和地衣。质坚硬，不易折断。横切面皮部窄，红棕色，纤维性强。木部宽，浅棕色，有多数细孔状导管。髓部小，黑褐色，呈空洞状。具特异香气，味苦而涩。
Slices circular, elliptical or irregular in shape, 1.8-6.5 cm in diameter. Surface greyish-brown, dark purple-red at places where cork has peeled off; cork thick, coarsely cracked in some, fissured; in others, longitudinally grooved, often with mosses and lichens attached. Texture hard, not easily broken. Transverse section: cork narrow, reddish-brown, fibrous; wood wide, light brown, with numerous pitted vessels; pith small, blackish-brown, hollow. Odour, distinctive; taste, bitter and astringent.

鉴别 Identification

（1）本品粉末暗红色。嵌晶纤维成束或散在，末端渐尖，直径21～62μm，壁极厚，胞腔不明显，壁中嵌有众多细小草酸钙方晶，有的方晶突出于胞壁表面。嵌晶石细胞不规则形或长椭圆形，直径38～92μm，壁厚，壁中嵌有众多细小草酸钙方晶。纤维管胞成束或散在。木栓细胞表面观多角形，垂周壁平直、菲薄；侧面观长方形。分泌细胞椭圆形，胞腔大，连有薄壁细胞碎片。导管为具缘纹孔导管，多破碎。棕色块散在，棕红色或棕色。
The powder is dark red. Crystal fibers are bundled or scattered, gradually pointed at the end, with a diameter of 21-62μm, extremely thick walls, indistinct cell cavities, and numerous small calcium oxalate crystals embedded in the walls, some of which protrude from the cell wall surface. Crystal stone cells are irregular or elongated, with a diameter of 38-92μm, thick walls, and numerous small calcium oxalate crystals embedded in the walls. Fiber vessel cells are bundled or scattered. Cork cells have a polygonal surface view, with straight and thin radial walls; side view is rectangular. Secretory cells are elliptical with large cell cavities, connected with thin-walled cell fragments. Vessels are ring-porous vessels, mostly broken. Brown blocks are scattered, reddish-brown or brown.

（2）取本品粉末0.5g，加环己烷10ml，超声处理30分钟，滤过，滤液蒸干，残渣加环己烷0.5ml使溶解，作为供试品溶液。另取异型南五味子丁素对照品，加环己烷制成每1ml含1mg的溶液，作为对照品溶液。照薄层色谱法（通则0502）试验，吸取上述两种溶液各5μl，分别点于同一硅胶GF254薄层板上，以石油醚（60～90℃）-乙酸乙酯（2∶1）为展开剂，展开，取出，晾干，置紫外光灯（254nm）下检视。供试品色谱中，在与对照品色谱相应的位置上，显相同颜色的斑点。
To 0.5g of the powder add 10ml of cyclohexane, treat with ultrasound for 30 minutes, filter, evaporate the filtrate to dryness, dissolve the residue in 0.5ml of cyclohexane as the test solution. Take Denshiso Ding Su as the reference substance, add cyclohexane to make a solution containing 1mg per ml as the reference solution. Carry out the method for thin layer chromatography<0502>, using silica gel GF254 as the coating substance and a mixture of petroleum ether (60-90°C) and ethyl acetate (2:1) as the mobile phase. Apply separately to the plate 5 μl of each of the above two solutions. After developing and removal of the plate, dry in air. Examine under ultraviolet light at 254 nm. The spot in the chromatogram obtained with the test solution corresponds in position and colour to the spot in the chromatogram obtained with the reference solution.

检查 Examination

水分 Water

不得过14.0%（通则0832第二法）。
Not more than 14.0 per cent <0832,method 2>.

总灰分 Total ash

不得过4.0%（通则2302）。
Not more than 4.0 per cent <2302>.

含量测定 Assay

照高效液相色谱法（通则0512）测定。
Carry out the assay by high performance liquid chromatography <0512>.

色谱条件与系统适用性试验 Chromatographic conditions and system suitability test

以十八烷基硅烷键合硅胶为填充剂；以甲醇-乙腈-水（10∶48∶42）为流动相；检测波长为230nm。理论板数按异型南五味子丁素峰计算应不低于8000。
Bonded silica gel with octadecylsilane as the filler; methanol-acetonitrile-water (10:48:42) as the mobile phase; detection wavelength at 230nm. The theoretical plate number calculated based on the peak of schisandrin C should not be less than 8000.

对照品溶液的制备 Preparation of reference solution

取异型南五味子丁素对照品适量，精密称定，加甲醇制成每1ml含30μg的溶液，即得。
Taking an appropriate amount of schisandrin C reference substance, accurately weigh it, and dissolve it in methanol to obtain a solution containing 30μg per 1ml.

供试品溶液的制备 Preparation of test solution

取本品粉末（过三号筛）约0.5g，精密称定，置具塞锥形瓶中，精密加入环己烷50ml，称定重量，超声处理（功率360W，频率40kHz）50分钟，放冷，再称定重量，用环己烷补足减失的重量，摇匀，滤过，精密量取续滤液25ml，蒸干，残渣加甲醇溶解，转移至5ml量瓶中，加甲醇至刻度，摇匀，滤过，取续滤液，即得。
Taking about 0.5g of the powder of this product (passed through a No. 3 sieve), accurately weigh it, place it in a stoppered conical flask, accurately add 50ml of hexane, weigh it, subject it to ultrasonic treatment (power 360W, frequency 40kHz) for 50 minutes, let it cool, weigh it again, make up for the weight loss with hexane, shake well, filter, accurately take 25ml of the filtrate, evaporate to dryness, dissolve the residue in methanol, transfer it to a 5ml volumetric flask, add methanol to the mark, shake well, filter, and take the filtrate.

测定法 Assay method

分别精密吸取对照品溶液与供试品溶液各10μl，注入液相色谱仪，测定，即得。
Precisely draw 10μl of the reference solution and the test solution, respectively, inject them into the liquid chromatograph, and determine.

本品按干燥品计算，含异型南五味子丁素（C27H30O8）不得少于0.050%。
Calculated on the dried product, the content of schisandrin C (C27H30O8) should not be less than 0.050%.

性 Property

温。
warm.

味 Flavor

苦、甘。
Bitter, sweet.

归经 Meridian tropism

归肝、肾经。
Liver and kidney meridians.

功能 Actions

活血补血，调经止痛，舒筋通络。
To promote blood circulation and nourish blood, regulate menstruation and relieve pain, and relax tendons and activate collaterals.

主治 Indications

用于月经不调，痛经，麻木瘫痪，风湿痹痛，气血虚弱。
Used for irregular menstruation, dysmenorrhea, numbness and paralysis, rheumatic arthralgia, and deficiency of qi and blood.

用量 Dosage

15~30g.
15-30g.

用法 Administration

无。
None.

贮藏 Storage

置干燥处。
Store in a dry place.

滇鸡血藤

文本参考：《中国药典（2015年版）》
Text reference: Chinese Pharmacopoeia (2015 Edition)

概述 Overview

本品为木兰科植物内南五味子Kadsura interior A. C. Smith的干燥藤茎。秋季采收，除去枝叶，切片，晒干。
Kadsura Stem is the dried lianoid stem of Kadsura interior A. C. Smith (Fam. Magnoliaceae). The drug is collected in autumn, removed from branch and leaf, cut into slices and dried in the sun.

性状 Description

本品呈圆形、椭圆形或不规则的斜切片，直径1.8～6.5cm。表面灰棕色，栓皮剥落处呈暗红紫色，栓皮较厚，粗者具多数裂隙，呈龟裂状；细者具纵沟，常附有苔类和地衣。质坚硬，不易折断。横切面皮部窄，红棕色_，纤维性强。木部宽，浅棕色，有多数细孔状导管。髓部小，黑褐色，呈空洞状。具特异香气，味苦而涩。
Oblique slices rounded, elliptical or irregular, 1.8-6.5 cm in diameter. Externally greyish-brown, dark reddish-purple where the cork exfoliated, cork relatively thick, the thick with numerous rimously cracked, the thin longitudinally furrowed, frequently covered with liverworts and lichens. Texture hard, uneasily broken. In transverse section, bark narrow, reddish-brown, strongly fibrous; wood broad, pale brown, with numerous minute-hole-shaped vessels. Pith small, blackish-brown, hollowed. Odour, aromatic, characteristic; taste, bitter and astringent.

鉴别 Identification

（1）本品粉末暗红色。嵌晶纤维成束或散在，末端渐尖，直径21～62μm，壁极厚，胞腔不明显，壁中嵌有众多细小草酸钙方晶，有的方晶突出于胞壁表面。嵌晶石细胞不规则形或长椭圆形，直径38～92μm，壁厚，壁中嵌有众多细小草酸钙方晶。纤维管胞成束或散在。木栓细胞表面观多角形，垂周壁平直、菲薄；侧面观长方形。分泌细胞椭圆形，胞腔大，连有薄壁细胞碎片。导管为具缘纹孔导管，多破碎。棕色块散在，棕红色或棕色。
（2）取本品粉末0.5g，加环己烷10ml，超声处理30分钟，滤过，滤液蒸干，残渣加环己烷0.5ml使溶解，作为供试品溶液。另取异型南五味子丁素对照品，加环己烷制成每1ml含1mg的溶液，作为对照品溶液。照薄层色谱法（通则0502）试验，吸取上述两种溶液各5μl，分别点于同一硅胶GF254薄层板上，以石油醚（60～90℃）-乙酸乙酯（2：1）为展开剂，展开，取出，晾干，置紫外光灯（254mn）下检视。供试品色谱中，在与对照品色谱相应的位置上，显相同颜色的斑点。

检查 Examination

水分 Water

不得过14.0%（通则0832第二法）。
Not more than 14.0 per cent <0832, method 2>.

总灰分 Total ash

不得过4.0%（通则2302）。
Not more than 4.0 per cent <2302>.

含量测定 Assay

照高效液相色谱法（通则0512）测定。
Carry out the method for high performance liquid chromatography <0512>.

色谱条件与系统适用性试验 Chromatography system and system suitability

以十八烷基硅烷键合硅胶为填充剂；以甲醇-乙腈-水（10：48：42）为流动相；检测波长为230mn。理论板数按异型南五味子丁素峰计算应不低于8000。
Use octadecylsilane bonded silica gel as the stationary phase and a mixture of methanol, acetonitrile and water (10 : 48 : 42) as the mobile phase. As detector a spectrophotometer set at 230 nm. The number of theoretical plates of the column is not less than 8000, calculated with reference to the peak of heteroclitin D.

对照品溶液的制备 Reference solution

取异型南五味子丁素对照品适量，精密称定，加甲醇制成每1ml含30μg的溶液，即得。
Dissolve a quantity of heteroclitin D CRS, accurately weighed, in methanol to produce a solution containing 30 μg per ml.

供试品溶液的制备 Test solution

取本品粉末（过三号筛）约0.5g，精密称定，置具塞锥形瓶中，精密加入环己烷50ml，称定重量，超声处理（功率360W，频率40kHz）50分钟，放冷，再称定重量，用环己烷补足减失的重量，摇匀，滤过，精密量取续滤液25ml，蒸干，残渣加甲醇溶解，转移至5ml量瓶中，加甲醇至刻度，摇匀，滤过，取续滤液，即得。
Weigh accurately 0.5 g of the powder (through No. 3 sieve) to a stoppered conical flask, add accurately 50 ml of cyclohexane, weigh, ultrasonicate (power 360 W, frequency 40 kHz) for 50 minutes, cool, weigh again and compensate the loss of the weight with cyclohexane, mix well and filter. Measure accurately 25 ml of the successive filtrate and evaporate to dryness. Dissolve the residue with methanol, transfer to a 5 ml volumetric flask and dilute to volume with methanol, mix well and filter, use the successive filtrate as the test solution.

测定法 Procedure

分别精密吸取对照品溶液与供试品溶液各10μl，注入液相色谱仪，测定，即得。
Inject accurately 10 μl of each of the reference solution and the test solution, respectively, into the column, and calculate the content.

本品按干燥品计算，含异型南五味子丁素（C27H30O8）不得少于0.050%。
It contains not less than 0.050 per cent of heteroclitin D (C27H30O8), calculated with reference to the dried drug.

性味与归经 Property and Flavor

苦、甘，温。归肝、肾经。
Warm; bitter, sweet. Liver and kidney meridians.

功能与主治 Actions

活血补血，调经止痛，舒筋通络。
To activate the blood, nourish blood, regulate menstruation, relieve pain, relax sinews, and unblock the collaterals.

主治 Indications

用于月经不调，痛经，麻木瘫痪，风湿痹痛，气血虚弱。
Menstrual irregularities, dysmenorrhea, numbness, paralysis, painful arthralgia caused by wind-dampness, deficiency of blood and qi.

用法与用量 Administration and dosage

15～30g。
15-30 g.

贮藏 Storage

置干燥处。
Preserve in a dry place.