This product is the dried leaves of the plant Callicarpa formosana Rolfe, a species in the Verbenaceae family. The leaves are harvested during the summer and autumn when the branches and leaves are lush, and then dried.
NMM ID | nmm-01wj |
Systematic Name | Callicarpa pedunculata Leaf |
Systematic Chinese Name | 杜虹花叶（dù hóng huā yè） |
Generic Name | Zi-zhu-ye |
Generic Chinese Name | 紫珠叶（zǐ zhū yè） |
NMM Type | plant |
Species Origins | Callicarpa pedunculata | 杜虹花 |
Medicinal Parts | leaf | 叶 |
Special Descriptions | |
Processing Methods | |
Systematic Name Explanation | This product is the dried leaves of the plant Callicarpa formosana Rolfe, a species in the Verbenaceae family. The leaves are harvested during the summer and autumn when the branches and leaves are lush, and then dried. |
Generic Name Explanation | NMMGN follows the Chinese name of the relevant Chinese medicinal materials in the Chinese Pharmacopoeia: 2020 edition: Volume I. |
Parent NMM | |
Child NMMs | |
Creators | |
Reviewers |
紫珠叶
English text reference: Chinese Pharmacopoeia (2020 Edition)
Overview
Callicarpa Leaf is the dried leaf of Callicarpa formosana Rolfe (Fam. Verbenaceae). The drug is collected in summer and autumn when the branches and leaves are luxuriant, removed from impurities, and dried.
Description
Leaves crumpled and curled, some broken. When the intact leaf is flattened, it is ovate-elliptic or elliptic, 4-19cm long, 2.5-9cm wide. Apex gradually acute or obtuse-rounded, base broadly cuneate or obtuse-rounded, margin finely serrate, nearly entire near the base. Upper surface grayish-green or brownish-green, with stellate hairs and short coarse hairs; lower surface pale green or pale brownish-green, densely covered with yellow-brown stellate hairs and golden glandular dots, prominent midrib and lateral veins, minor veins extending to the teeth. Petiole 0.5-1.5cm long. Odour, slight; taste, slightly bitter and astringent.
Identification
（1）The powder is grayish-yellow to brownish-brown. There are two types of non-glandular hairs: one is stellate hairs, mostly broken and lignified, with 1 to several rounds of 1-6 lateral cells in each round; the other non-glandular hairs are 1-3 cells in diameter, with thicker walls. The head of glandular scale consists of 8-11 cells, flat and spherical, with a very short stalk. The head of small glandular hair consists of 2-4 cells, with a stalk of 1-2 cells. Calcium oxalate cluster crystals are small and scattered in the leaf parenchyma cells.
（2）Take 1g of the powder, add 30ml of ether, heat under reflux for 30 minutes, filter, evaporate the filtrate to dryness, dissolve the residue in 2ml of methanol, and take the supernatant as the test solution. Take ursolic acid CRS, add methanol to make a solution containing 1mg per ml as the reference solution. Carry out the method for thin layer chromatography<0502>, using silica gel G as the coating substance and a mixture of cyclohexane, chloroform, ethyl acetate, and glacial acetic acid (20:5:8:0.1) as the mobile phase. Apply separately to the plate 3-5μl of the test solution and 3μl of the reference solution. After developing and removal of the plate, dry in air. Spray with a 10% solution of sulfuric acid in ethanol, heat at 105°C to the spots clear. The spot in the chromatogram obtained with the test solution corresponds in position and colour to the spot in the chromatogram obtained with the reference solution.
Examination
Water
Not more than 15.0 per cent <0832,method 2>.
Total ash
Not more than 11.0 per cent <2302>.
Extractives
Carry out the method for determination of ethanol-soluble Extractives <2201,the hot maceration method>, using diluted ethanol as the solvent,not less than 20.0 per cent.
Content determination
Carry out the method for determination of content by high performance liquid chromatography <0512>.
Chromatographic conditions and system suitability test
Use octadecylsilane bonded silica gel as the filler; use acetonitrile-0.5% phosphoric acid solution (17:83) as the mobile phase; detect at a wavelength of 332nm. The theoretical plate number calculated based on the peak of astragalin should not be less than 3000.
Preparation of reference solution
Take an appropriate amount of astragalin reference substance, accurately weigh, add 50% methanol to make a solution containing 50μg per ml.
Preparation of test solution
Take about 0.25g of the powder of this product (passed through a No. 4 sieve), accurately weigh, place it in a stoppered conical flask, accurately add 50% methanol 50ml, seal tightly, weigh, let it stand overnight, heat reflux for 1 hour, cool, weigh again, make up for the weight loss with 50% methanol, shake well, filter, and take the filtrate.
Determination method
Accurately take 10μl of the reference solution and the test solution, respectively, inject into the liquid chromatograph, and determine.
Calculated on the dried product, the content of astragalin (C29H36O15) should not be less than 0.50%.
Prepared slices
Processing
Eliminate Foreign matter,wash, cut into sections, and dry in the sun.
Property
Cold.
Flavor
Bitter and astringent.
Meridian tropism
Liver, lung, and stomach meridians.
Actions
To cool the blood, astringe and stop bleeding, disperse stasis, detoxify, and reduce swelling.
Indications
Used for bleeding, hemoptysis, vomiting blood, blood in the stool, metrorrhagia and metrostaxis, bleeding from external injuries, heat-toxin sores and ulcers, and scalds.
Dosage
3-15g. For external use, apply an appropriate amount.
Administration
Grind into powder and take orally 1.5-3g. Apply to the affected area.
Storage
Store in a well-ventilated and dry place.
紫珠叶
Text reference: Chinese Pharmacopoeia (2015 Edition)
Overview
Callicarpa Formosana Leaf is the dried leaf of Callicarpa formosana Rolfe (Fam. Verbenaceae). The drug is collected in summer and autumn when foliage branch growing luxuriantly, and dried.
Description
Mostly crumpled and rolled, some broken. When whole, ovate-elliptical or elliptical, 4-19 cm long, 2.5-9 cm wide. Apex acuminate or obtuse-rounded, base broadly cuneate or obtuse-rounded, margin serrulate, entire near the base. Upper surface greyish-green or brownish green, covered with stellate hairs and dumpy hairs; lower surface pale green or pale brownish-green, densely covered with yellowish brown stellate hairs and golden-yellow glandular dots, midrib and lateral veins prominent, veinlets extending to the end of serra. Petiols 0.5-1.5 cm long. Odour, slight; taste, slightly bitter and astringent.
Identification
（2）取本品粉末1g，加乙醚30ml，加热回流30分钟，滤过，滤液蒸干，残渣加甲醇2ml使溶解，取上清液作为供试品溶液。另取熊果酸对照品，加甲醇制成每1ml含1mg的溶液，作为对照品溶液。照薄层色谱法（通则0502）试验，吸取供试品溶液3～5μl、对照品溶液3μl，分别点于同一硅胶G薄层板上，以环己烷-三氯甲烷-乙酸乙酯-冰醋酸（20：5：8：0.1）为展开剂，展开，取出，晾干，喷以10%硫酸乙醇溶液，在105℃加热至斑点显色清晰。供试品色谱中，在与对照品色谱相应的位置上，显相同颜色的斑点。
Examination
Water
Not more than 15.0 per cent <0832, method 2>.
Total ash
Not more than 11.0 per cent <2302>.
Extractives
Carry out the method for determination of ethanol-soluble extractives <2201, the hot extraction method>, using dilute ethanol as the solvent, not less than 20.0 per cent.
Assay
Carry out the method for high performance liquid chromatography <0512>.
Chromatographic system and system suitability
Use octadecylsilane bonded silica gel as the stationary phase and a mixture of acetonitrile and 0.5% phosphoric acid (17:83) as the mobile phase. The detection wavelength is 332 nm. The theoretical plate number calculated with reference to the peak of verbascoside should not be less than 3000.
Preparation of reference solution
Dissolve a quantity of verbascoside CRS, accurately weighed, in 50% methanol to prepare a solution containing 50 μg per ml.
Preparation of test solution
Weigh accurately 0.25 g of the powder (through No. 4 sieve) to a stoppered conical flask, add accurately 50 ml of 50% methanol, stopper tightly, weigh and allow to stand overnight. Heat under reflux for 1 hour, cool, weigh again, replenish the loss of the weight with 50% methanol, mix well and filter. Use the successive filtrate as the test solution.
Procedure
Inject accurately 10 μl of each of the reference solution and the test solution, respectively, into the column, and calculate the content.
It contains not less than 0.50 per cent of verbascoside (C29H36O15), calculated with reference to the dried drug.
Prepared slices
Callicarpa Formosana Leaf
Processing
Eliminate foreign matter, wash clean, cut into sections, and dry.
Property
Cool.
Flavor
Bitter, astringent.
Meridian tropism
Liver, lung, and stomach meridians.
Actions
To cool the blood, astringe and stanch bleeding, dissipate stasis, remove toxin, and relieve swelling.
Indications
Epistaxis, hemoptysis, hematemesis, bloody stool, menstrual flooding and spotting, traumatic bleeding, sore and ulcer caused by heat toxin, scald and burn.
Dosage
3-15 g. Ground into powder for oral administration: 1.5-3 g. Appropriate amount for topical application.
Storage
Preserve in a ventilated and dry place.
This product is the dried leaves of the plant Callicarpa formosana Rolfe, a species in the Verbenaceae family. The leaves are harvested during the summer and autumn when the branches and leaves are lush, and then dried.