This product is the dried mature fruit of the plant Perilla frutescens (L.) Britt., a member of the mint family. The fruit is harvested in autumn when it is ripe, impurities are removed, and then it is dried in the sun.
NMM ID | nmm-01vx |
Systematic Name | Perilla frutescens Fruit |
Systematic Chinese Name | 紫苏果实（zǐ sū guǒ shí） |
Generic Name | Zi-su-zi |
Generic Chinese Name | 紫苏子（zǐ sū zǐ） |
NMM Type | plant |
Species Origins | Perilla frutescens | 紫苏 |
Medicinal Parts | fruit | 果实 |
Special Descriptions | |
Processing Methods | |
Systematic Name Explanation | This product is the dried mature fruit of the plant Perilla frutescens (L.) Britt., a member of the mint family. The fruit is harvested in autumn when it is ripe, impurities are removed, and then it is dried in the sun. |
Generic Name Explanation | NMMGN follows the Chinese name of the relevant Chinese medicinal materials in the Chinese Pharmacopoeia: 2020 edition: Volume I. |
Parent NMM | |
Child NMMs | |
Creators | |
Reviewers |
紫苏子
English text reference: Chinese Pharmacopoeia (2020 Edition)
Overview
Perilla Fruit is the dried mature fruit of Perilla frutescens (L.) Britt. (Fam. Labiatae). The drug is collected in autumn when the fruit is mature, removed from impurities, and dried in the sun.
Description
The fruit is ovate or nearly spherical, about 1.5mm in diameter. The surface is grayish-brown or grayish-brown, with slightly raised dark purple reticulate veins. The base is slightly pointed, with grayish-white dotted fruit stalk scars. The fruit peel is thin and brittle, easily crushed. The seeds are yellowish-white, with membranous seed coat, and the cotyledons are 2, whitish, and oily. It has a fragrant aroma when crushed and a slightly pungent taste.
Identification
(1)This product is powdered gray-brown. The cross-sectional view of the epidermal cells of the seed coat is extremely flattened, with hook-like thickened walls; The surface is oval-shaped, and the walls are densely carved and hook-like thickened. The exocarp cells are yellowish-brown, the cross-sectional cells are flattened, and the outer wall is papillae; The surface is round-like, with slightly curved walls and a fine keratin texture on the surface. The endocarp tissue is mainly a heterotype stone cell in cross-section, which is irregularly shaped; The top view is polygonal, with unclear intercellular boundaries and a stellate cavity. Inner endosperm cells vary in size and contain fatty oil droplets; Some contain fine calcium oxalate cubic crystals. Cotyledons are rectangular in shape and filled with fatty oil droplets.
(2)Take 1g of powder of this product, add 25ml of methanol, sonicate for 30 minutes, filter, evaporate the filtrate, and add 1ml of methanol to the residue to dissolve it as a test solution. In addition, 1g of perilla seeds were taken as a control medicinal material, and a control medicinal material solution was made by the same method. According to the thin layer chromatography <2302> test, 2μl of each of the above two solutions was absorbed, which were respectively placed on the same silica gel G thin layer plate, and n-hexane-toluene-ethyl acetate-formic acid (2:5:2.5:0.5) was used as the developing solvent, expanded, taken out, dried, sprayed with aluminum trichloride test solution, and placed under ultraviolet light (365nm) for inspection. In the chromatography of the test sample, spots of the same color are displayed at the corresponding position of the chromatography of the control medicinal material.
Examination
Water
Not more than 8.0 per cent <0832, method 2>.
Content determination
It was determined by high performance liquid chromatography <0512>.
Chromatographic conditions and system suitability test
Octadecylsilane bonded silica gel is used as filler; methanol-0.1% formic acid solution (40∶60) was used as mobile phase; The detection wavelength is 330nm. The number of theoretical plates should not be less than 3000 according to the calculation of the rosmarinic acid peak.
Preparation of reference solution
Take an appropriate amount of rosmarinic acid reference substance, weigh it accurately, and add methanol to make a solution containing 80μg per ml.
Preparation of test solution
Take about 0.5g of the powder of this product (through the second sieve), weigh it accurately, place it in an Erlenmeyer flask with a stopper, add 50ml of 80% methanol accurately, plug it, weigh it, heat and reflux it for 2 hours, let it cool, weigh it again, make up for the weight loss with 80% methanol, shake it well, filter it, and take the filtrate.
Determination method
10μl of the reference solution and 20μl of the test solution were accurately absorbed, injected into the liquid chromatograph, and measured.
This product is calculated as a dry product, and the content of rosmarinic acid (C18H16O8) shall not be less than 0.25%.
Prepared slices
Perilla Fruit
Processing
Remove impurities, wash and dry.
Description
Same as the crude drug.
Identification
Same as the crude drug.
Examination
Same as the crude drug.
Content determination
Same as the crude drug.
Fried Perilla Fruit
Processing
Take Perilla Fruit and fry them according to the clear stir-fry method <0213> until there is a burst sound.
Description
This product is shaped like perilla seeds, the surface is gray-brown, there are fine cracks, and there is a burnt aroma.
Examination
Water
Same as the crude drug. Not more than 2.0 per cent.
Content determination
Same as the crude drug. Contains rosmarinic acid (C18H16O8) not less than 0.20%.
Identification
Same as the crude drug.
Property
Warm.
Flavor
Pungent.
Meridian tropism
Lung meridian.
Actions
Lowers gas and dissolves phlegm, relieves cough and asthma, moistens the intestines and laxatives.
Indications
Used for phlegm, qi reversal, cough and asthma, intestinal dryness and constipation.
Dosage
3-10g.
Administration
None.
Storage
Place in a ventilated and dry place to prevent moths.
紫苏子
Text reference: Chinese Pharmacopoeia (2015 Edition)
Overview
Perilla Fruit is the dried ripe fruit of Perilla frutescens (L) Britt. (Fam. Labiatae). The drug is collected in autumn when ripe, removed from foreign matter, and dried in the sun.
Description
Ovoid or subspherical, about 1.5 mm in diameter. Externally greyish-brown, with dark purple and slightly protuberant reticulate striations, base slightly acute, with greyish-white and pointed fruit stalk scar. Pericarp thin and fragile, easily broken. Seeds yellowish-white, testa membranous, cotyledons 2, whitish and oily. Odour, aromatic by pressing taste, slightly pungent.
Identification
（2）取本品粉末1g，加甲醇25ml，超声处理30分钟，滤过，滤液蒸干，残渣加甲醇1ml使溶解，作为供试品溶液。另取紫苏子对照药材1g，同法制成对照药材溶液。照薄层色谱法（通则0502）试验，吸取上述两种溶液各2μl，分别点于同一硅胶G薄层板上，以正己烷-甲苯-乙酸乙酯-甲酸（2：5：2.5：0.5）为展开剂，展开，取出，晾干，喷以三氯化铝试液，置紫外光灯（365nm）下检视。供试品色谱中，在与对照药材色谱相应的位置上，显相同颜色的斑点。
Examination
Water
Not more than 8.0 per cent <0832, method 2>.
Assay
Carry out the method for high performance liquid chromatography <0512>.
以十八烷基硅烷键合硅胶为填充剂；以甲醇-0.1%甲酸溶液（40：60）为流动相；检测波长为330nm。理论板数按迷迭香酸峰计算应不低于3000。
取迷迭香酸对照品适量，精密称定，加甲醇制成每1ml含80μg的溶液，即得。
取本品粉末（过二号筛）约0.5g，精密称定，置具塞锥形瓶中，精密加入80%甲醇50ml，密塞，称定重量，加热回流2小时，放冷，再称定重量，用80%甲醇补足减失的重量，摇匀，滤过，取续滤液，即得。
分别精密吸取对照品溶液10μl与供试品溶液20μl，注入液相色谱仪，测定，即得。
It contains not less than 0.25 per cent of rosmarinic acid (C18H16O8), calculated with reference to the dried drug.
Prepared slices
Perilla Fruit
Processing
Eliminate foreign matter, wash clean, and dry.
Stir-baked Perilla Fruit
Stir-baked the clean Perilla Fruit as described under the method for simple stir-baking <0213> until the drug burst.
Similar to the crude drug in shape, externally greyish brown, with fine cracks. With charred scent.
同药材，不得过2.0%。
Assay It contains not less than 0.20 per cent of rosmarinic acid (C18H16O8), following the method for the crude drug.
Identification As required for the crude drug.
Property and Flavor Warm; pungent.
Actions To direct qi downward, resolve phlegm, suppress cough, relieve wheezing, moisten the intestines, and relax the bowels.
Administration and dosage 3-10 g.
Storage Preserve in a ventilated and dry place, and protect from moth.
This product is the dried mature fruit of the plant Perilla frutescens (L.) Britt., a member of the mint family. The fruit is harvested in autumn when it is ripe, impurities are removed, and then it is dried in the sun.