滇鸡血藤

English text reference: Chinese Pharmacopoeia (2020 Edition)

Overview

Kadsura Stem is the dried stem of Kadsura interior A. C. Smith (Fam. Magnoliaceae). The drug is collected in autumn, removed from branches and leaves, sliced, and dried.

Description

Slices circular, elliptical or irregular in shape, 1.8-6.5 cm in diameter. Surface greyish-brown, dark purple-red at places where cork has peeled off; cork thick, coarsely cracked in some, fissured; in others, longitudinally grooved, often with mosses and lichens attached. Texture hard, not easily broken. Transverse section: cork narrow, reddish-brown, fibrous; wood wide, light brown, with numerous pitted vessels; pith small, blackish-brown, hollow. Odour, distinctive; taste, bitter and astringent.

Identification

The powder is dark red. Crystal fibers are bundled or scattered, gradually pointed at the end, with a diameter of 21-62μm, extremely thick walls, indistinct cell cavities, and numerous small calcium oxalate crystals embedded in the walls, some of which protrude from the cell wall surface. Crystal stone cells are irregular or elongated, with a diameter of 38-92μm, thick walls, and numerous small calcium oxalate crystals embedded in the walls. Fiber vessel cells are bundled or scattered. Cork cells have a polygonal surface view, with straight and thin radial walls; side view is rectangular. Secretory cells are elliptical with large cell cavities, connected with thin-walled cell fragments. Vessels are ring-porous vessels, mostly broken. Brown blocks are scattered, reddish-brown or brown.

To 0.5g of the powder add 10ml of cyclohexane, treat with ultrasound for 30 minutes, filter, evaporate the filtrate to dryness, dissolve the residue in 0.5ml of cyclohexane as the test solution. Take Denshiso Ding Su as the reference substance, add cyclohexane to make a solution containing 1mg per ml as the reference solution. Carry out the method for thin layer chromatography<0502>, using silica gel GF254 as the coating substance and a mixture of petroleum ether (60-90°C) and ethyl acetate (2:1) as the mobile phase. Apply separately to the plate 5 μl of each of the above two solutions. After developing and removal of the plate, dry in air. Examine under ultraviolet light at 254 nm. The spot in the chromatogram obtained with the test solution corresponds in position and colour to the spot in the chromatogram obtained with the reference solution.

Examination

Water

Not more than 14.0 per cent <0832,method 2>.

Total ash

Not more than 4.0 per cent <2302>.

Assay

Carry out the assay by high performance liquid chromatography <0512>.

Chromatographic conditions and system suitability test

Bonded silica gel with octadecylsilane as the filler; methanol-acetonitrile-water (10:48:42) as the mobile phase; detection wavelength at 230nm. The theoretical plate number calculated based on the peak of schisandrin C should not be less than 8000.

Preparation of reference solution

Taking an appropriate amount of schisandrin C reference substance, accurately weigh it, and dissolve it in methanol to obtain a solution containing 30μg per 1ml.

Preparation of test solution

Taking about 0.5g of the powder of this product (passed through a No. 3 sieve), accurately weigh it, place it in a stoppered conical flask, accurately add 50ml of hexane, weigh it, subject it to ultrasonic treatment (power 360W, frequency 40kHz) for 50 minutes, let it cool, weigh it again, make up for the weight loss with hexane, shake well, filter, accurately take 25ml of the filtrate, evaporate to dryness, dissolve the residue in methanol, transfer it to a 5ml volumetric flask, add methanol to the mark, shake well, filter, and take the filtrate.

Assay method

Precisely draw 10μl of the reference solution and the test solution, respectively, inject them into the liquid chromatograph, and determine.

Calculated on the dried product, the content of schisandrin C (C27H30O8) should not be less than 0.050%.

Property

warm.

Flavor

Bitter, sweet.

Meridian tropism

Liver and kidney meridians.

Actions

To promote blood circulation and nourish blood, regulate menstruation and relieve pain, and relax tendons and activate collaterals.

Indications

Used for irregular menstruation, dysmenorrhea, numbness and paralysis, rheumatic arthralgia, and deficiency of qi and blood.

Dosage

15-30g.

Administration

None.

Storage

Store in a dry place.

滇鸡血藤

Text reference: Chinese Pharmacopoeia (2015 Edition)

Overview

Kadsura Stem is the dried lianoid stem of Kadsura interior A. C. Smith (Fam. Magnoliaceae). The drug is collected in autumn, removed from branch and leaf, cut into slices and dried in the sun.

Description

Oblique slices rounded, elliptical or irregular, 1.8-6.5 cm in diameter. Externally greyish-brown, dark reddish-purple where the cork exfoliated, cork relatively thick, the thick with numerous rimously cracked, the thin longitudinally furrowed, frequently covered with liverworts and lichens. Texture hard, uneasily broken. In transverse section, bark narrow, reddish-brown, strongly fibrous; wood broad, pale brown, with numerous minute-hole-shaped vessels. Pith small, blackish-brown, hollowed. Odour, aromatic, characteristic; taste, bitter and astringent.

Identification

（2）取本品粉末0.5g，加环己烷10ml，超声处理30分钟，滤过，滤液蒸干，残渣加环己烷0.5ml使溶解，作为供试品溶液。另取异型南五味子丁素对照品，加环己烷制成每1ml含1mg的溶液，作为对照品溶液。照薄层色谱法（通则0502）试验，吸取上述两种溶液各5μl，分别点于同一硅胶GF254薄层板上，以石油醚（60～90℃）-乙酸乙酯（2：1）为展开剂，展开，取出，晾干，置紫外光灯（254mn）下检视。供试品色谱中，在与对照品色谱相应的位置上，显相同颜色的斑点。

Examination

Water

Not more than 14.0 per cent <0832, method 2>.

Total ash

Not more than 4.0 per cent <2302>.

Assay

Carry out the method for high performance liquid chromatography <0512>.

Chromatography system and system suitability

Use octadecylsilane bonded silica gel as the stationary phase and a mixture of methanol, acetonitrile and water (10 : 48 : 42) as the mobile phase. As detector a spectrophotometer set at 230 nm. The number of theoretical plates of the column is not less than 8000, calculated with reference to the peak of heteroclitin D.

Reference solution

Dissolve a quantity of heteroclitin D CRS, accurately weighed, in methanol to produce a solution containing 30 μg per ml.

Test solution

Weigh accurately 0.5 g of the powder (through No. 3 sieve) to a stoppered conical flask, add accurately 50 ml of cyclohexane, weigh, ultrasonicate (power 360 W, frequency 40 kHz) for 50 minutes, cool, weigh again and compensate the loss of the weight with cyclohexane, mix well and filter. Measure accurately 25 ml of the successive filtrate and evaporate to dryness. Dissolve the residue with methanol, transfer to a 5 ml volumetric flask and dilute to volume with methanol, mix well and filter, use the successive filtrate as the test solution.

Procedure

Inject accurately 10 μl of each of the reference solution and the test solution, respectively, into the column, and calculate the content.

It contains not less than 0.050 per cent of heteroclitin D (C27H30O8), calculated with reference to the dried drug.

Property and Flavor

Warm; bitter, sweet. Liver and kidney meridians.

Actions

To activate the blood, nourish blood, regulate menstruation, relieve pain, relax sinews, and unblock the collaterals.

Indications

Menstrual irregularities, dysmenorrhea, numbness, paralysis, painful arthralgia caused by wind-dampness, deficiency of blood and qi.

Administration and dosage

15-30 g.

Storage

Preserve in a dry place.